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Detection of SINEUP RNA modification by Oxford Nanopore direct-RNA sequencing

ABSTRACT: To detect the modifed bases in SINEUP RNA, we compared chemically modified in vitro transcribed (IVT) SINEUP-GFP RNA and in-cell transcribed (ICT) SINEUP RNA from SINEUP-GFP and sense EGFP co-transfected HEK293T/17 cells. Comparative study of Nanopore direct RNA sequencing data from non-modified and modified IVT samples against the data from ICT SINEUP RNA sample revealed modified k-mers positions in SINEUP RNA in the cell.

ORGANISM(S): Homo sapiens

PROVIDER: GSE224018 | GEO | 2023/02/04

REPOSITORIES: GEO

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