Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA sequencing of skeletal muscle macrophages

ABSTRACT: To investigate the functional heterogeneity of muscle macrophages, we sorted 3 macrophage populations from 4wk-old WT and mdx mice based on the expression of Gal3 (gal-3+ Mac), FOLR2 (SkMRM) and CD52 (MDM). We then performed gene expression profiling analysis using data obtained from RNA-seq of the FACs-sorted populations of muscle macrophages.

ORGANISM(S): Mus musculus

PROVIDER: GSE225593 | GEO | 2023/05/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

PGE2-induced OSM expression

Project description:The objective of the study was to compare the wound macs with corresponding macs derived from peripheral blood monocytes (MDMs). Wound site macrophage (wound macs were isolated from human subjects with chronic wounds. Matching blood monocyte derived macrophages (MDM) were obtained from same subjects. Transcriptome profiling (GeneChip, Affymetrix) was performed.The expression values of genes were normalized using global scaling approach. Blood monocyte derived macrophages or human wound macrophages were isolated and transcriptome analysis was performed using affymetrix gene chip analysis. Group -1 MDMs (n=3) Mac-1 Mac-2 Mac-3 Group -2 Wound macs (n=3) Wmac-1 Wmac-2 Wmac-3

2012-08-01 | E-GEOD-36995 | biostudies-arrayexpress

GATA6 dependent gene regulation in peritoneal macrophages

Project description:Peritoneal macrophages from control and Mac-Gata6 KO (LysM-cre;Gata6-floxed) mice were determined for genome wide gene expression. Sorted peritoneal macrophages from control and Mac-Gata6 KO mice were performed for whole genome expression analysis by Illumina microarray

2014-05-02 | E-GEOD-56684 | biostudies-arrayexpress

Muscle injury induces a transient senescence-like state that is required for myofiber growth during muscle regeneration

Project description:Cellular senescence is the irreversible arrest of normally dividing cells and is driven by the cell cycle inhibitors Cdkn2a, Cdkn1a, and Trp53. Senescent cells are implicated in chronic diseases and tissue repair through their increased secretion of proinflammatory factors known as the senescence-associated secretory phenotype (SASP). Here, we use spatial transcriptomics and single-cell RNA sequencing (scRNAseq) to demonstrate that cells displaying senescent characteristics are “transiently" present within regenerating skeletal muscle and within the muscles of D2-mdx mice, a model of Muscular Dystrophy. Following injury, multiple cell types including macrophages and fibro-adipogenic progenitors (FAPs) upregulate senescent features such as senescence pathway genes, SASP factors, and senescence-associated beta-gal (SA-β-gal) activity. Importantly, when these cells were removed with ABT-263, a senolytic compound, satellite cells are reduced, and muscle fibers were impaired in growth and myonuclear accretion.

2022-10-11 | GSE214892 | GEO

Muscle injury induces a transient senescence-like state that is required for myofiber growth during muscle regeneration [Spatial Transcriptomics]

2022-10-14 | GSE215305 | GEO

RNA-seq of genetically engineered mouse EGFR positive glioblastoma tumors with PDGFRA and GAL-1 positive and negative populations

Project description:RNA-seq of genetically engineered mouse EGFR positive glioblastoma tumors flow cytometry sorted into PDGFRA and GAL-1 positive and negative populations.

2022-04-06 | GSE195813 | GEO

Tissue-resident FOLR2+ macrophages associate with tumor-infiltrating CD8+ T cells and with increased survival of breast cancer patients

Project description:Macrophage infiltration is a hallmark of solid cancers and overall macrophage infiltration correlates with lower patient survival and resistance to therapy. Tumor- associated macrophages, however, are phenotypically and functionally heterogeneous. Specific subsets of tumor-associated macrophage might be endowed with antagonistic roles on cancer progression and on the development of anti-tumor immunity. Here, we identify a discrete population of FOLR2 + tissue-resident macrophages in healthy mammary gland and breast cancer primary tumors. FOLR2 + macrophages localize in perivascular areas in the tumor stroma, where they interact with CD8 + T cells. Moreover, FOLR2 + macrophages efficiently prime effector CD8 + T cells ex vivo . The density of FOLR2 + macrophages in tumors positively correlates with better patient survival. This study highlights antagonistic roles for tumor-associated macrophage subsets and paves the way for subset-specific therapeutic interventions in macrophages-based cancer therapies.

2022-01-06 | GSE192935 | GEO

Pleural CD14+ monocytes/macrophages of healthy adolescents showa high expression of metallothionein family genes

Project description:High quality CD14+ monocytes/macrophages (plMo/Mφ) were used for RNA-sequencing (RNA-seq) in comparison with human monocyte-derived macrophages (MDM) natural (MDM-0) or IL-4-polarized(MDM-IL4)

2023-09-06 | GSE217995 | GEO

TLR4 is a regulator of trained immunity in a murine model of Duchennemuscular dystorphy

Project description:The main purpose of our study is to investigate if muscle damage in mdx mice alters the phenotype of macropahges at precursor level in the bone marrow. Here we show major remodeling of the epigenetic, metabolic and functional inflammatory profiles of monocyte-derived macrophages from the bone marrow of mdx mice is TLR4-dependent. This altered phenotype of mdx BMDM demonstrates the hallmark features of trained immunity, including being transmissible and long-lasting after bone marrow transplantation to non-dystrophic mice.

2021-12-07 | GSE190307 | GEO

Effects of Treg depletion in dystrophic muscle.

Project description:CD4+Foxp3+ regulatory T cells (Tregs) accumulate in skeletal muscle from dystrophin-deficient mdx mice. Analysis of global gene expression in muscles from mdx mice treated with anti-CD25 compared with muscles from mdx mice treated with control antibody revealed that Tregs partially protect mdx mice from muscle pathology and promote muscle repair/regeneration.

2013-12-15 | GSE50095 | GEO

Gsα regulates macrophage foam cell formation during atherosclerosis

Project description:To investigate in vivo transcriptome profiles of macrophages from CTR and Mac-GsαKO mice , we isolated and cultured peritoneal macrophages. Our data indicates that macrophages from CTR and Mac-GsαKO mice show different mRNA expressions. Compared with macrophages from CTR mice, macrophages from Mac-GsαKO mice expressed more genes related to lipid metabolism.

2024-01-06 | GSE252233 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data