ATAC-seq analysis of various murine B cell subsets resulting from different immunogens.
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ABSTRACT: Purpose: We aimed to link different variations of memory B cell subsets to their proliferating precursors via shared epigenetic features. Methods : Spleens were processed according to the manuscript. Cells were sorted and DNA was isolated using a protocol adapted from Corces et al. Samples were sequenced to obtain 20M 2x75 bp paired-end reads using an Illumina NextSeq 550 sequencer (Illumina, Inc, California, USA). Results: Memory cells have many DARs in comparison to FO naïve cells. However, differences between memory cells themselves can be attributed to imprinting by their respective proliferating precursors. A germinal center B cell program may be required to establish some DARs in GC-derived memory cells.
ORGANISM(S): Mus musculus
PROVIDER: GSE225672 | GEO | 2024/01/07
REPOSITORIES: GEO
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