Dataset Information

Sex differences in the rewiring of brainstem miRNA regulatory networks driving hypertension development

ABSTRACT: In this study, we elucidated the role of miRNA dysregulation in brainstem autnomic circuits as hypertension develops. Female spontaneously hypertensive rats (SHR) and normotensive wistar kyoto rats were sacrified at 8, 10, 12, 16, and 24 weeks of age. 8 weeks is considered prior to the onset of hypertension while 10 and 12 weeks correspond to the age of onset. At 16 weeks hypertension has developed and at 24 weeks is considered chronic persistent hypertension. Three autonomic nuclei in the brainstem were collected, the nucleus of the solitary tract (NTS), the caudal ventrolateral medulla (CVLM), and rostral ventrolateral medulla (RVLM). NanoString rat miRNA panel was used to obtain miRNA expression profiles from all collected samples.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE227762 | GEO | 2025/06/12

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:The rostral ventrolateral medulla (RVLM), a part of the medullary reticular formation, plays a major role in several physiological responses, including cardiovascular and sympathetic nervous system functions. Although aging causes disturbances in the responses of these physiological systems, RVLM involvement in these age-related changes is not clear. Previous work using high-throughput gene expression analysis of the RVLM in aged animals suggested that chemical neurotransmission-related genes might be downregulated with advancing age. Since the RVLM function involves a balance of inhibitory and excitatory inputs, which is largely mediated by gamma-aminobutyric acid (GABA) and excitatory amino acid (EAA) neurotransmission, we hypothesized that aging is associated with altered excitatory and/or inhibitory neurotransmission-related gene expression in the RVLM. To test this hypothesis, we micropunched an RVLM-containing area from young (3–5 months), middle-aged (12–14 months), and aged (22–26 months) Fischer 344 male rats. RNA purified from these micropunches was analyzed using GABA and Glutamate RT2 Profiler PCR arrays (n= 8–10). In addition, the expression of selected genes was validated at the RNA level using TaqMan® based- qPCR and at the protein level using western blotting. All the genes that displayed significant differential expression (1.5-fold, p < .05, FDR < .05) were identified to be downregulated in the RVLM of aged and middle-aged rats compared to young rats. Among the downregulated genes, the percentage of glutamate neurotransmission-related genes was higher than GABA neurotransmission-related genes. Solute carrier family 1 member 6 (Slc1a6) gene showed the highest fold downregulation at the RNA level in the RVLM of aged compared to young rats, and its protein product, Excitatory amino acid transporter 4 (EAAT4), showed a downregulatory trend in the RVLM of aged and middle-aged rats. These results suggest that molecular constituents of both GABA and glutamate neurotransmission might be altered in the RVLM of aged and middle-aged rats, and the changes in glutamate neurotransmission might be more prominent. Investigating age-associated anatomical and functional changes in RVLM GABA and glutamate neurotransmission might provide a foundation for understanding the effects of aging on physiological function.

Dataset Information

Sex differences in the rewiring of brainstem miRNA regulatory networks driving hypertension development

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets