Dataset's files
| Action | DRS | |||
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| GSE228265_RAW.tar | Raw | |||
| GSE228265_allcountsum.txt.gz | Txt | |||
| filelist.txt | Txt |
Items per page: 5 1 - 4 of 4 |
Similar Datasets
Project description:Peg3 is an imprinted gene that in itself is a KRAB zinc finger protein. We established knockout trophoblast stem cell lines (TSCs) in which we ablated the majority of the zinc finger motifs of the PEG3 coding region using CRISPR-Cas9-mediated deletion. We then profiled wild-type (WT) and knockout (KO) TSC lines for the genome-wide distribution of the epigenetic repressive mark H3K9me3 by ChIP-seq.
2026-06-19 | GSE331039 | GEO
Project description:Investigating the impact of Padi2/3 deletion on trophoblast stem cells.
2022-08-09 | GSE208305 | GEO
Project description:Trophectoderm-specific expression of Angiomotin (AMOT) in pre-implantation embryos followed by its unique expression in the post-implantation ectoplacental cone that harbors the trophoblast stem cell niche prompted our investigation on the function of AMOT in trophoblast cells. Using the in vitro trophoblast stem cell culture model, we established differentiation dependent up-regulation of AMOT expression in trophoblast cells. To understand the function of AMOT in trophoblast cells mass spectrometry-based proteomic analysis was employed to identify the AMOT interactome within the trophoblast proteome. This approach utilized immunoprecipitation of endogenous AMOT followed by fractionation on SDS-PAGE and subsequently subjecting the tryptic digested excised gel bands to mass spectrometry.
2021-11-03 | PXD019391 | Pride
Project description:PADI function in trophoblast stem cells
| PRJNA859160 | ENA
Project description:As medical abortion has established itself as the significant method of terminating pregnancies, many studies in recent years has focused on Mifepristone, a principal agent used in this field. It can be found that much effort has been devoted to the research on the impact of Mifepristone on the decidua and embryo implantation process in the mechanism of abortion and contraception; the mechanism of its impact on trophoblast cells, however, still remains unclear. Therefore, the present study aims to fill the gap by investigating the effects of Mifepristone on villous trophoblasts in medical abortion patients. We have found that Mifepristone affects trophoblast cell proliferation and invasion through the progesterone receptor. We established PGR-knockdown trophoblast stem cells and collected these cells for comprehensive RNA-seq analysis.
2025-10-26 | GSE265928 | GEO
Project description:Eomesodermin (Eomes) interacting partners via RIME in mouse trophoblast stem cells
2025-05-14 | MSV000097887 | MassIVE
Project description:Mesoangioblasts are vessel-associated progenitor cells that show therapeutic promise for the treatment of muscular dystrophy. Mesoangioblasts have the ability to undergo skeletal muscle differentiation and cross the blood vessel wall regardless of the developmental stage at which they are isolated. Here we show that PW1/Peg3 is expressed at high levels in mesoangioblasts obtained from mouse, dog and human tissues and its level of expression correlates with their myogenic competence. Silencing PW1/Peg3 markedly inhibits myogenic potential of mesoangioblasts in vitro through MyoD degradation. Moreover, lack of PW1/Peg3 abrogates mesoangioblast ability to cross the vessel wall and to engraft into damaged myofibers through the modulation of the junctional adhesion molecule-A. We conclude that PW1/Peg3 function is essential for conferring proper mesoangioblast competence and that the determination of PW1/Peg3 levels in human mesoangioblasts may serve as a biomarker to identify the best donor populations for therapeutic application in muscular dystrophies.
2015-01-19 | GSE64334 | GEO
Project description:Mesoangioblasts are vessel-associated progenitor cells that show therapeutic promise for the treatment of muscular dystrophy. Mesoangioblasts have the ability to undergo skeletal muscle differentiation and cross the blood vessel wall regardless of the developmental stage at which they are isolated. Here we show that PW1/Peg3 is expressed at high levels in mesoangioblasts obtained from mouse, dog and human tissues and its level of expression correlates with their myogenic competence. Silencing PW1/Peg3 markedly inhibits myogenic potential of mesoangioblasts in vitro through MyoD degradation. Moreover, lack of PW1/Peg3 abrogates mesoangioblast ability to cross the vessel wall and to engraft into damaged myofibers through the modulation of the junctional adhesion molecule-A. We conclude that PW1/Peg3 function is essential for conferring proper mesoangioblast competence and that the determination of PW1/Peg3 levels in human mesoangioblasts may serve as a biomarker to identify the best donor populations for therapeutic application in muscular dystrophies. Ctrl Mesoangioblasts (MABs) transduced with a Lentiviral vector shControl (2 replicates, Ctrl_1 and Ctrl_2) and shPW1 Mesoangioblasts transduced with a Lentiviral vector shPW1 (2 replicates, PW1-siRna_1 and PW1-siRna_2)
2015-01-19 | E-GEOD-64334 | biostudies-arrayexpress
Project description:PEG3 (Paternally Expressed Gene 3) is an imprinted gene encoding a DNA-binding protein. Two components of the mammalian MSL (Male-Specific Lethal) complex, Msl1 and Msl3, are known to be the genomic targets of PEG3. In this report, we performed genome-wide ChIP-seq experiments profiling H4K16ac levels using a set of MEF cells (Mouse Embryonic Fibroblast), the WT and KO of Peg3. The results indicated that about 10% of the mouse gene catalog showed high levels of H4K16ac levels in Peg3-KO MEFs than in Peg3-WT MEFs. This further suggests that mammalian Peg3 may control its downstream genes through the MSL complex.
2017-08-15 | GSE97459 | GEO
Project description:PEG3 (Paternally Expressed Gene 3) is an imprinted gene encoding a DNA-binding protein. In this study, we used MEF cells (Mouse Embryonic Fibroblast). We performed genome-wide ChIP-seq experiments profiling H3K9me3 levels as well as examine the set of genomic targets and also redefines the DNA-binding motif for the imprinted transcription factor, PEG3 in the WT and KO of Peg3. The results suggested high levels of H3K9 levels in Peg3-KO MEFs than in Peg3-WT MEFs, whereas several different genes involved several biochemical pathways seems to be have potential binding site for PEG3 protein, which tend to have a distinct profile of binding motif to its binding region. This further suggests that mammalian Peg3 may control its downstream genes in regulating several biological functions and regulate the H3K9 methylation levels.
2021-07-31 | GSE181069 | GEO