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MPRA in primary human macrophages

ABSTRACT: We investigated an intergenic haplotype on chr21q22, linked to five different inflammatory diseases. We used a functional approach (massively-parallel reporter assay; MPRA) to first identify active enhancers at the locus in primary human macrophages, and then determine if candidate variants within these regulatory regions might alter enhancer activity. In doing so, we discovered a mechanism that orchestrates macrophage responses during chronic inflammation and delineated how the risk haplotype increases expression of the causal gene, ETS2.

ORGANISM(S): synthetic construct Homo sapiens

PROVIDER: GSE229472 | GEO | 2024/05/01

REPOSITORIES: GEO

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