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Effect of meiosis specific cohesin subunit SMC1 on chromatin architecture in primary murine spermatocytes

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE230556 | GEO | 2023/05/04

REPOSITORIES: GEO

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Effect of meiosis specific cohesin subunit SMC1 on chromatin architecture in primary murine spermatocytes [ATAC-Seq]

Project description:To investigate the function of meiosis-specific SMC1beta on chromatin architecture near the telomeric end in primary murine spermatocytes we used a SMC1beta knockout mouse model.

2023-05-04 | GSE230553 | GEO

Effect of meiosis specific cohesin subunit SMC1 on chromatin architecture in primary murine spermatocytes [RNA-Seq 2]

2023-05-04 | GSE230555 | GEO

Effect of meiosis specific cohesin subunit SMC1 on chromatin architecture in primary murine spermatocytes [RNA-Seq 1]

2023-05-04 | GSE230554 | GEO

Effect of meiosis specific cohesin subunit SMC1 on chromatin architecture in primary murine spermatocytes

Project description:Effect of meiosis specific cohesin subunit SMC1 on chromatin architecture in primary murine spermatocytes

| PRJNA961750 | ENA

Effect of meiosis specific cohesin subunit SMC1 on chromatin architecture in primary murine spermatocytes [ATAC-Seq]

Project description:Effect of meiosis specific cohesin subunit SMC1 on chromatin architecture in primary murine spermatocytes [ATAC-Seq]

| PRJNA961756 | ENA

Effect of meiosis specific cohesin subunit SMC1 on chromatin architecture in primary murine spermatocytes [RNA-Seq 1]

Project description:Effect of meiosis specific cohesin subunit SMC1 on chromatin architecture in primary murine spermatocytes [RNA-Seq 1]

| PRJNA961757 | ENA

Effect of meiosis specific cohesin subunit SMC1 on chromatin architecture in primary murine spermatocytes [RNA-Seq 2]

Project description:Effect of meiosis specific cohesin subunit SMC1 on chromatin architecture in primary murine spermatocytes [RNA-Seq 2]

| PRJNA961758 | ENA

Novel meiosis-specific isoform of mammalian SMC1.

Project description:Structural maintenance of chromosomes (SMC) proteins fulfill pivotal roles in chromosome dynamics. In yeast, the SMC1-SMC3 heterodimer is required for meiotic sister chromatid cohesion and DNA recombination. Little is known, however, about mammalian SMC proteins in meiotic cells. We have identified a novel SMC protein (SMC1beta), which-except for a unique, basic, DNA binding C-terminal motif-is highly homologous to SMC1 (which may now be called SMC1alpha) and is not present in the yeast genome. SMC1beta is specifically expressed in testes and coimmunoprecipitates with SMC3 from testis nuclear extracts, but not from a variety of somatic cells. This establishes for mammalian cells the concept of cell-type- and tissue-specific SMC protein isoforms. Analysis of testis sections and chromosome spreads of various stages of meiosis revealed localization of SMC1beta along the axial elements of synaptonemal complexes in prophase I. Most SMC1beta dissociates from the chromosome arms in late-pachytene-diplotene cells. However, SMC1beta, but not SMC1alpha, remains chromatin associated at the centromeres up to metaphase II. Thus, SMC1beta and not SMC1alpha is likely involved in maintaining cohesion between sister centromeres until anaphase II.

| S-EPMC99874 | biostudies-literature

Cohesin subunit SMC1 associates with mitotic microtubules at the spindle pole.

Project description:Accurate mitotic chromosome segregation depends on the formation of a microtubule-based bipolar spindle apparatus. We report that the cohesin subunit structural maintenance of chromosomes subunit 1 (SMC1) is recruited to microtubule-bound RNA export factor 1 (Rae1) at the mitotic spindle pole. We locate the Rae1-binding site to a 21-residue-long region, SMC1(947-967) and provide several lines of evidence that phosphorylation of Ser(957) and Ser(966) of SMC1 stimulates binding to Rae1. Imbalances in these assembly pathways caused formation of multipolar spindles. Our data suggest that cohesin's known bundling function for chromatids in mitotic and interphase cells extends to microtubules at the spindle pole.

| S-EPMC2557025 | biostudies-literature

Aging predisposes oocytes to meiotic nondisjunction when the cohesin subunit SMC1 is reduced.

Project description:In humans, meiotic chromosome segregation errors increase dramatically as women age, but the molecular defects responsible are largely unknown. Cohesion along the arms of meiotic sister chromatids provides an evolutionarily conserved mechanism to keep recombinant chromosomes associated until anaphase I. One attractive hypothesis to explain age-dependent nondisjunction (NDJ) is that loss of cohesion over time causes recombinant homologues to dissociate prematurely and segregate randomly during the first meiotic division. Using Drosophila as a model system, we have tested this hypothesis and observe a significant increase in meiosis I NDJ in experimentally aged Drosophila oocytes when the cohesin protein SMC1 is reduced. Our finding that missegregation of recombinant homologues increases with age supports the model that chiasmata are destabilized by gradual loss of cohesion over time. Moreover, the stage at which Drosophila oocytes are most vulnerable to age-related defects is analogous to that at which human oocytes remain arrested for decades. Our data provide the first demonstration in any organism that, when meiotic cohesion begins intact, the aging process can weaken it sufficiently and cause missegregation of recombinant chromosomes. One major advantage of these studies is that we have reduced but not eliminated the SMC1 subunit. Therefore, we have been able to investigate how aging affects normal meiotic cohesion. Our findings that recombinant chromosomes are at highest risk for loss of chiasmata during diplotene argue that human oocytes are most vulnerable to age-induced loss of meiotic cohesion at the stage at which they remain arrested for several years.

| S-EPMC2577922 | biostudies-literature

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