Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Targeting RhoA nuclear mechanoactivity rejuvenates aged hematopoietic stem cells

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE233989 | GEO | 2025/08/29

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Targeting RhoA nuclear mechanoactivity rejuvenates aged hematopoietic stem cells [scRNA-seq]

Project description:Biomechanical alterations contribute to the decreased regenerative capacity of hematopoietic stem cells (HSCs) upon aging. RhoA is a key regulator of mechanosignaling, but its role in mechanotransduction in stem cell aging remains unclear. Here we show that murine HSCs respond to increased nuclear envelope (NE) tension by inducing NE translocation of P-cPLA2, which cell-intrinsically activates RhoA. Aged HSCs experience physiologically higher intrinsic NE tension, but reducing RhoA activity lowers NE tension in aged HSCs. Feature image analysis of HSC nuclei reveals that chromatin remodeling is associated with RhoA inhibition, including restoration of youthful levels of the heterochromatin marker H3K9me2 and a decrease in chromatin accessibility and transcription at retrotransposons. Finally, we demonstrate that RhoA inhibition upregulates Klf4 expression and transcriptional activity, improving aged HSC regenerative capacity and lympho/myeloid skewing in vivo. Together, our data outline an intrinsic RhoA-dependent mechanosignaling axis, which can be pharmacologically targeted to restore aged stem cell function.

2025-08-29 | GSE269953 | GEO

Targeting RhoA nuclear mechanoactivity rejuvenates aged hematopoietic stem cells [bulk ATAC-seq]

2025-08-29 | GSE233986 | GEO

Targeting RhoA nuclear mechanoactivity rejuvenates aged hematopoietic stem cells [bulk RNA-seq]

2025-08-29 | GSE233985 | GEO

Targeting RhoA nuclear mechanoactivity rejuvenates aged hematopoietic stem cells [bulk ATAC-seq confinement]

2025-08-29 | GSE269396 | GEO

Targeting RhoA nuclear mechanoactivity rejuvenates aged hematopoietic stem cells

Project description:Targeting RhoA nuclear mechanoactivity rejuvenates aged hematopoietic stem cells

| PRJNA979153 | ENA

Targeting RhoA nuclear mechanoactivity rejuvenates aged hematopoietic stem cells [scRNA-seq]

Project description:Targeting RhoA nuclear mechanoactivity rejuvenates aged hematopoietic stem cells [scRNA-seq]

| PRJNA1124165 | ENA

Targeting RhoA nuclear mechanoactivity rejuvenates aged hematopoietic stem cells [bulk RNA-seq]

Project description:Targeting RhoA nuclear mechanoactivity rejuvenates aged hematopoietic stem cells [bulk RNA-seq]

| PRJNA979157 | ENA

Targeting RhoA nuclear mechanoactivity rejuvenates aged hematopoietic stem cells [bulk ATAC-seq]

Project description:Targeting RhoA nuclear mechanoactivity rejuvenates aged hematopoietic stem cells [bulk ATAC-seq]

| PRJNA979159 | ENA

Targeting RhoA nuclear mechanoactivity rejuvenates aged hematopoietic stem cells [bulk ATAC-seq confinement]

Project description:Targeting RhoA nuclear mechanoactivity rejuvenates aged hematopoietic stem cells [bulk ATAC-seq confinement]

| PRJNA1121374 | ENA

Endothelial transplantation rejuvenates aged hematopoietic stem cell function.

Project description:Age-related changes in the hematopoietic compartment are primarily attributed to cell-intrinsic alterations in hematopoietic stem cells (HSCs); however, the contribution of the aged microenvironment has not been adequately evaluated. Understanding the role of the bone marrow (BM) microenvironment in supporting HSC function may prove to be beneficial in treating age-related functional hematopoietic decline. Here, we determined that aging of endothelial cells (ECs), a critical component of the BM microenvironment, was sufficient to drive hematopoietic aging phenotypes in young HSCs. We used an ex vivo hematopoietic stem and progenitor cell/EC (HSPC/EC) coculture system as well as in vivo EC infusions following myelosuppressive injury in mice to demonstrate that aged ECs impair the repopulating activity of young HSCs and impart a myeloid bias. Conversely, young ECs restored the repopulating capacity of aged HSCs but were unable to reverse the intrinsic myeloid bias. Infusion of young, HSC-supportive BM ECs enhanced hematopoietic recovery following myelosuppressive injury and restored endogenous HSC function in aged mice. Coinfusion of young ECs augmented aged HSC engraftment and enhanced overall survival in lethally irradiated mice by mitigating damage to the BM vascular microenvironment. These data lay the groundwork for the exploration of EC therapies that can serve as adjuvant modalities to enhance HSC engraftment and accelerate hematopoietic recovery in the elderly population following myelosuppressive regimens.

| S-EPMC5663355 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data