Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human iPSC-derived pulmonary epithelial cells infected with SARS-CoV-2 variants in the micro-patterned culture plates [SARS-CoV-2]

ABSTRACT: We infected iPSC-derived pulmonary epithelial cells in the micro-patterned culture plate with the SARS-CoV-2 variants. Analyses of infected alveolar and airway epithelial cells, respectively, revealed the tropism of the variants.

ORGANISM(S): Homo sapiens

PROVIDER: GSE236841 | GEO | 2024/02/29

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

The iPSC-derived pulmonary epithilial cells in micro-patterned culture plate

Project description:We established a micro-patterned respiratory epithelial cell culture system in vitro. In this culture system, various types of lung epithelial cells were identified.

2024-02-29 | GSE249762 | GEO

Micro-patterned culture of iPSC-derived airway epithelial cells [airway]

Project description:We established a micro-patterned respiratory epithelial cell culture system in vitro. In this culture system, the iPSC-derived lung progenitor cells were differentiated into airway epithelial cells in a position-specific manner.

2024-02-29 | GSE236840 | GEO

Micro-patterned culture of iPSC-derived alveolar epithelial cells [alveolar]

Project description:We established a micro-patterned respiratory epithelial cell culture system in vitro. In this culture system, the iPSC-derived lung progenitor cells were differentiated into alveolar epithelial cells in a position-specific manner.

2024-02-29 | GSE236839 | GEO

Phenotype modulation of VSMCs on patterned substrate

Project description:Loss of contractility and acquisition of an epithelial phenotype of vascular smooth muscle cells (VSMCs) are key events in proliferative vascular pathologies such as atherosclerosis and post-angioplastic restenosis. There is no proper cell culture system allowing VSMC differentiation so that it is difficult to delineate the molecular mechanism responsible for proliferative vasculopathy. We investigated whether a micro-patterned substrate could restore the contractile phenotype of VSMCs in vitro. To induce and maintain the differentiated VSMC phenotype in vitro, we introduced a micro-patterned groove substrate to modulate the morphology and function of VSMCs.

2013-11-14 | E-MTAB-1888 | biostudies-arrayexpress

SARS-CoV-2 infection of Lung Organoids Reveals Conserved Use of Tetraspanin-8 by Ancestral-, Delta-, and Omicron- Variants

Project description:Ancestral SARS coronavirus-2 (SARS-CoV-2) and variants of concern (VOC) caused a global pandemic with a spectrum of disease variation linked to immune dysfunction. The mechanistic underpinnings of variation related to lung epithelium are relatively understudied. Here, we biobanked lung organoids by preserving stem cell function. We optimized viral infection with H1N1 swine flu and next comprehensively characterized epithelial responses to SARS-CoV-2 infection in phenotypically stable lung organoids from twenty different subjects. We discovered Tetraspanin 8 (TSPAN8) as a novel mediator of SARS-CoV-2-infection. TSPAN8 facilitates SARS-CoV-2 infection rates but does not via enhanced ACE-2-mediated entry. In head-to-head comparisons with Ancestral SARS-CoV-2, Delta- and Omicron- VOC displayed lower overall infection rates of organoids but triggered increased epithelial interferon responses. All variants shared highest tropism for ciliated- and goblet- cells. ACE2- and TSPAN8- expression are universal features of infected cells. TSPAN8-blocking antibodies diminish SARS-CoV-2 infection and may spur novel avenues for COVID-19 therapy.

2022-09-30 | GSE211562 | GEO

Transcriptomes of human iPSC-derived alveolar and airway cells in the micro-patterned culture plates and their responses to SARS-CoV-2 variant infection

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-02-29 | GSE236842 | GEO

Single-cell RNA-sequencing of human bronchial epithelial organoids infected by four different betacoronaviruses; HCoV-OC43, SARS-CoV, MERS-CoV, and SARS-CoV-2 and mock control.

Project description:We investigated the interactions of four distinct betacoronaviruses; HCoV-OC43, SARS-CoV, MERS-CoV, and SARS-CoV-2 within human bronchial epithelial (HBE) organoids using single-cell RNA sequencing (scRNA-seq) to comprehensively understand betacoronaviruses cellular tropism and the intricate interplay between these cells and the host's immune defense mechanisms.

2024-03-26 | GSE262439 | GEO

iPSC-derived Human Intestinal Organoids are permissive to SARS-CoV-2 infection

Project description:We performed transcriptomic profiling of cells derived from human induced pluripotent stem cells (iPSCs) using a directed differentiation protocol to generate mesenchyme free, regionally patterned intestinal organoids. These organoids were then infected with SARS-CoV-2, and sequencing was performed 1 and 4 days post infection

2021-04-20 | GSE159201 | GEO

Limited extent and consequences of pancreatic SARS-CoV-2 infection

Project description:Several studies have suggested a relationship between SARS-CoV-2 infection and diabetes. This study examined the consequences of infection of human pancreatic islets with SARS-CoV-2 virus. This GEO submission contains the raw and processed data from single-cell RNA sequencing (scRNAseq) experiments evaluating the tropism of SARS-CoV-2 in pancreatic islets and transcriptional changes induced by infection of these cells. Overall we observed limited infection of pancreatic islets (0.2 - 3.4% of all cells infected per donor) and identified multiple pancreatic cell types as targets of infection; due to the preponderance of major endocrine cell populations in our islet cell preparations, downstream analyses were primarily focused on alpha and beta cells. Within beta cells we identified an upregulation of interferon stimulated genes in both infected and bystander cells as well as an NFκB mediated genes in infected cells only. Within alpha cells we detected a non-specific downregulation of a large number of host genes in infected cells.

2022-01-23 | GSE194061 | GEO