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C/EBP⍺ Aggravated Ferroptosis and Apoptosis in Renal Tubular Epithelial Cell

ABSTRACT: Acute Kidney Injury (AKI) is widely recognized for its rapid deterioration in renal function, often resulting in unfavorable clinical outcomes. An increase in the expression of CCAAT/enhancer binding protein alpha (C/EBP⍺) has been observed in both human and mouse kidneys affected by AKI. We unraveled the downstream pathway of C/EBP⍺ through RNA-sequencing, confirming our findings with qRT-PCR and luciferase reporter assays. Furthermore, we identified a potential target for AKI reversal in a murine AKI model.

ORGANISM(S): Homo sapiens

PROVIDER: GSE242804 | GEO | 2023/09/14

REPOSITORIES: GEO

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2018-06-11 | GSE99607 | GEO

C/EBP? and PPAR?-specific transcriptional programs in mature adipocytes in vivo

Project description:We took a systematic approach to determine the transcriptional programs that are specifically regulated by C/EBP? in mature white adipocytes of mice on chow diet or high fat diet. The hypothesis tested in the present study was that C/EBP?, as a lipogenic transcription factor, has unique direct targets compared to PPAR?. Our inducible adipocyte specific knockout system allows us to test the direct targets of C/EBP? and PPAR? in adipocytes by short-term C/EBP? or PPAR? elimination in mature adipocytes in vivo. Results indicate that although it has been shown that C/EBP? and PPAR? cross-regulate each other, they have distinct direct responsive targets. Moreover, there are very few C/EBP? specific targets in mice on a chow diet, most of the C/EBP? targets in mature adipocytes are genes modulated by HFD feeding. Total RNA obtained from subcutaneous adipose tissue of Adn-C/EBP?-/- mice on doxycycline chow diet for 3 days, doxycycline high fat diet for 3 days or 1 month and Adn-PPAR?-/- mice on doxycycline chow diet for 3 days, compared to control littermates.

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Synchronized renal tubular cell death involves ferroptosis.

Project description: Not available

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