Dataset Information


P. aeruginosa PA14 mutant PA4496 biofilm vs wild-type biofilm

ABSTRACT: P. aeruginosa PA14 mutant strain PA4496 expression in biofilm cells relative to PA14 wild-type strain expression in biofilm cells. All samples cultured in LB with glass wool Overall design: Strains: P. aeruginosa PA14 wild-type, mutant PA4496 Medium: LB Biofilm grown glass wool Time: 7 hr Cell type: biofilm

INSTRUMENT(S): [Pae_G1a] Affymetrix Pseudomonas aeruginosa Array

SUBMITTER: Thomas K. Wood  

PROVIDER: GSE24638 | GEO | 2018-02-14


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Substrate Binding Protein DppA1 of ABC Transporter DppBCDF Increases Biofilm Formation in Pseudomonas aeruginosa by Inhibiting Pf5 Prophage Lysis.

Lee Yunho Y   Song Sooyeon S   Sheng Lili L   Zhu Lei L   Kim Jun-Seob JS   Wood Thomas K TK  

Frontiers in microbiology 20180124

Filamentous phage impact biofilm development, stress tolerance, virulence, biofilm dispersal, and colony variants. Previously, we identified 137 Pseudomonas aeruginosa PA14 mutants with more than threefold enhanced and 88 mutants with more than 10-fold reduced biofilm formation by screening 5850 transposon mutants (PLoS Pathogens5: e1000483, 2009). Here, we characterized the function of one of these 225 mutations, dppA1 (PA14_58350), in regard to biofilm formation. DppA1 is a substrate-binding p  ...[more]

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