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Genome wide gene expression profiles of Drosophila l(3)mbt larval brains and cultured tumors

ABSTRACT: Mutants in the Drosophila gene lethal (3) malignant brain tumor cause malignant growth in the larval brain. This data shows the changes in gene expression profile associated to mutations in l(3)mbt, both in situ in third instar larval brains and in tumors cultured for 1 5 and 10 (T1, T5, T10) rounds of allograft culture

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE24917 | GEO | 2011/02/01

SECONDARY ACCESSION(S): PRJNA132109

REPOSITORIES: GEO

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Genome wide gene expression profiles of Drosophila l(3)mbt larval brains and cultured tumors

Project description:Mutants in the Drosophila gene lethal (3) malignant brain tumor cause malignant growth in the larval brain. This data shows the changes in gene expression profile associated to mutations in l(3)mbt, both in situ in third instar larval brains and in tumors cultured for 1 5 and 10 (T1, T5, T10) rounds of allograft culture We performed genome-wide gene expression profiling of l(3)mbt[E2] and l(3)mbt[ts1] homozygous and transheterozygous larval brains raised at restrictive temperatures (29º C). We obtained three replicates for l(3)mbt[E2] at 17ªC (MBT_E2_17), l(3)mbt[ts1] at 17ºC (MBT_TS1_17), l(3)mbt[E2] at 29ºC (MBT_E2_29) and the transheterozygous at 29ºC (MBT_TS1_ME2), and six replicates l(3)mbt[ts1] at 29ºC (MBT_TS1_29). We also obtained three replicates for l(3)mbt[ts1] tumors at the first (T1), fifth (T5) and tenth (T10) round of allograft culture in adult flies, and six replicates for wild type w[1118] flies (WT).

2011-02-01 | E-GEOD-24917 | biostudies-arrayexpress

Genome wide gene expression profiles of Drosophila metastatic cultured tumors

Project description:Clones in the intestinal adult stem cell population mutant for Apc and Apc2, and over-expressing RasV12 and Snail are able to disseminate outside the gut and form macrometastases. These macrometastases can be cultured in allografts. This data shows the changes in gene expression profile of culturde tumors after 1, 2 and 10 (T1, T2, T10) rounds of allograft transplantation

2019-04-30 | GSE125312 | GEO

The tumor suppressor L(3)mbt inhibits neuroepithelial proliferation and acts on insulator elements

Project description:In Drosophila, defects in asymmetric cell division can result in the formation of stem cell derived tumors. Here, we reveal a different mechanism that can result in the formation of very similar terminal brain tumor phenotypes. We demonstrate that brain tumors in l(3)mbt mutants originate from overproliferation of neuroepithelial cells in the optic lobes caused by de-repression of target genes in the Salvador-Warts-Hippo (SWH) pathway. We use ChIP-seq to identify L(3)mbt-binding sites and show that L(3)mbt binds to chromatin insulator elements. Mutating l(3)mbt or inhibiting the insulator protein mod(mdg4) results in upregulation of SWH pathway reporters. As l(3)mbt tumors are rescued by mutations in bantam or yorkie or by overexpression of expanded the deregulation of SWH pathway target genes is an essential step in brain tumor formation. Our data reveal that very different primary defects can result in the formation of brain tumors, which behave quite similarly in their advanced stages. Examination of l(3)mbt binding in third instar larval brain and wing/haltere/third leg imaginal discs tissue.

2011-08-29 | E-GEOD-29206 | biostudies-arrayexpress

The tumor suppressor L(3)mbt inhibits neuroepithelial proliferation and acts on insulator elements

2011-08-29 | GSE29206 | GEO

Distribution of Drosophila insulator proteins Mod(mdg4) and L(3)mbt in Kc cells

Project description:Here we map the localization of Mod(mdg4), including Mod(mdg4)2.2 specific and Mod(mdg4) common to all isoforms, to chromatin insulators, as well as the lethal-3 malignant brain tumor protein in Drosophila Kc cells. examination of genomic occupancy for Mod(mdg4)2.2, Mod(mdg4)BTB (all isoforms), and L(3)mbt, control samples used for Drosophila Kc were previously described (Wood, Van Bortle et al., 2011 GSE30740)

2012-06-18 | E-GEOD-36393 | biostudies-arrayexpress

All Tumor Sector Experiments (T1-T20)

Project description:This SuperSeries is composed of the following subset Series: GSE16598: Tumor T1 Sectors GSE16599: Tumor T2 Sectors GSE16600: Tumor T3 Sectors GSE16601: Tumor T4 Sectors GSE16602: Tumor T5 Sectors GSE16603: Tumor T6 Sectors GSE16604: Tumor T7 Sectors GSE16605: Tumor T8 Sectors GSE16606: Tumor T9 Sectors GSE16607: Tumor T10 Sectors GSE16608: Tumor T11 Sectors GSE16609: Tumor T12 Sectors GSE16610: Tumor T13 Sectors GSE16611: Tumor T14 Sectors GSE16663: Tumor T15 Sectors GSE16664: Tumor T16 Sectors GSE16665: Tumor T17 Sectors GSE16667: Tumor T18 Sectors GSE16668: Tumor T19 Sectors GSE16669: Tumor T20 Sectors ROMA CGH experiments were conducted on four to six dissected sectors (S1-S6) from single primary ductal carcinomas (T1-T20). T1-T4 were analyzed by sectoring and analysis by ROMA. T5-T20 were analyzed using the Sector-Ploidy-Profiling (SPP) Approach. SPP involves macro-dissecting the tumor, flow-sorting nuclei by differences in total genomic DNA content and profiling the genome of the tumor subpopulations. The genomic DNA from each tumor was labeled with Cy5 and a reference fibroblast genome was labeled with Cy3 and cohybridized to a ROMA microarray (85K or 390K). Experiments were performed in color-reversal by dye-swapping samples and hybridizing the labeled DNA to a second microarray for T1-T15 experiments, but not for T16-T20 experiments.

2009-07-10 | E-GEOD-16672 | biostudies-arrayexpress

shotgun LC/MS/MS of L(3)mbt-IP samples using OSCs

Project description:L(3)mbt regulate the transcription of germ-specific genes in somatic cells but the detailed mechanism remains unclear. We performed shotgun LC/MS/MS of L(3)mbt-IP samples and identified a new interactor in OSCs.

2022-08-15 | PXD026945 | Pride

Transcription profiling of mouse fetal liver cells obtained from MBT-1+/+ or MBT-1-/- embryos at E14.5

Project description:Gene expression in the fetal liver cells obtained from MBT-1+/+ or MBT-1-/- embryos at E14.5 were analyzed using Affymetrix Genechip to assess the effect of the gene knock-out on hematopoietic cells.

2004-11-30 | E-MEXP-219 | biostudies-arrayexpress

Tissue-specific gene expression changes in l(3)mbt mutant ovaries [soma]

Project description:We performed RNAseq on l(3)mbt mutant somatic ovaries to gain a genome-wide view of tissue-specific gene expression changes in L(3)mbt-depleted somatic ovaries.

2018-02-01 | GSE108472 | GEO

Tissue-specific gene expression changes in l(3)mbt mutant ovaries [germline]

Project description:We performed RNAseq on embryos laid by somatically complemented l(3)mbt mutant germline to gain a genome-wide view of tissue-specific gene expression changes in L(3)mbt-depleted germline.

2018-02-01 | GSE108471 | GEO

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