Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Human-induced pluripotent Stem Cell-Derived Hepatic Stellate Cells in Liver Organoids: Maturation Promotion and Essential Role in Vascularization

ABSTRACT: Human-induced pluripotent stem cell (hiPSC)-derived liver organoids (LOs) are valuable for studying human liver organogenesis but face challenges in faithfully recapitulating certain processes, like vasculogenesis, due to the lack of specific cell components. Hepatic stellate cells (HSCs), which are liver-specific pericytes and might be crucial for liver vasculogenesis, remain underutilized in developmental studies because of their disease-related "activated" status and inefficient generation process. Here, we present an efficient method for generating hiPSC-derived HSCs (hiPSC-HSCs) resembling non-activated HSCs in the healthy human liver. These hiPSC-HSCs exhibit exceptional expandability (>105-fold) while maintaining essential cellular features. Additionally, in entirely hiPSC-derived LOs consisting of HSCs, hepatic endoderm, and endothelial cells, hiPSC-HSCs play a vital role in LO maturation and vascularization, both in vitro and in vivo. This work represents a significant advancement in understanding HSC roles in human liver development, and LOs containing non-activated hiPSC-HSCs hold potential in modeling congenital human liver diseases.

ORGANISM(S): Homo sapiens

PROVIDER: GSE249826 | GEO | 2026/03/20

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Human-induced pluripotent Stem Cell-Derived Hepatic Stellate Cells in Liver Organoids: Maturation Promotion and Essential Role in Vascularization [scRNA-Seq]

Project description:Human-induced pluripotent stem cell (hiPSC)-derived liver organoids (LOs) are valuable for studying human liver organogenesis but face challenges in faithfully recapitulating certain processes, like vasculogenesis, due to the lack of specific cell components. Hepatic stellate cells (HSCs), which are liver-specific pericytes and might be crucial for liver vasculogenesis, remain underutilized in developmental studies because of their disease-related "activated" status and inefficient generation process. Here, we present an efficient method for generating hiPSC-derived HSCs (hiPSC-HSCs) resembling fetal HSCs’ transcriptomic profiles. These hiPSC-HSCs exhibit exceptional expandability (>105-fold) while maintaining essential cellular features. Additionally, in entirely hiPSC-derived LOs consisting of HSCs, hepatic endoderm, and endothelial cells, hiPSC-HSCs play a vital role in LO maturation and vascularization, both in vitro and in vivo. This work represents a significant advancement in understanding HSC roles in human liver development, and LOs containing non-activated hiPSC-HSCs hold potential in modeling congenital human liver diseases.

2026-03-20 | GSE289592 | GEO

Transcriptome of human liver cells and culture-activated hepatic stellate cells

Project description:The molecular determinants of a healthy human liver cell phenotype remain largely uncharacterized. In addition, the gene expression changes associated with activation of primary human hepatic stellate cells, a key event during fibrogenesis, remain poorly characterized. Here, we provide the transriptomic profile underpinning the healthy phenotype of human hepatocytes, liver sinusoidal endothelial cells (LSECs) and quiescent hepatic stellate cells (qHSCs) as well as activated HSCs (aHSCs) We assess the transcriptome for purified, non-cultured human hepatocytes, liver sinusoidal cells (LSECs) and quiescent hepatic stellate cells (qHSCs) as well as culture-activated HSCs (aHSCs). Hepatocytes (n=2 donors), LSECs (n=3), qHSCs (n=3) and in vitro activated HSCs (n=3; from the same donors as the qHSCs and LSECs) were used for this study.

2016-03-02 | E-GEOD-68000 | biostudies-arrayexpress

Human iPSC-liver organoid transplant reduces fibrosis by immunomodulation

Project description:Donor organ shortage for transplant is a serious problem worldwide. Organoid culture technology, which allows the generation of complex organs from stem cells, provides a potential solution. We previously reported in vivo vascularization of human induced pluripotent stem cell (hiPSC)-derived liver buds (LBs) and subsequent improved survival of recipient mice with acute liver failure1,2. Here, we show that mid-gestational fetal liver tissue can generate de novo liver when grafted onto the surface of host livers and improve survival and liver functions in a model of liver failure. Next, we created complex fetal liver-like organoids (LOs) by a fusion of hiPSC-LBs to induce static cell-cell interactions, and show that these contain hepatocytes, vasculature, and bile duct after transplantation. Fused hiPSC-LOs show superior liver functions compared to hiPSC-LBs and improve survival and functions of liver failure model upon transplantation. Finally, transplant of fused hiPSC-LOs ameliorates liver fibrosis, a symptom of liver cirrhosis which leads to organ dysfunction, through their immunomodulatory effects especially on macrophage polarization that plays an important role in the regulation of liver fibrosis3–5. Taken together, our results suggest that hiPSC-LO transplant could be a promising therapeutic option for liver failure patients in the near future

2025-09-30 | GSE214648 | GEO

An in vitro human liver model by iPSC-derived parenchymal and non-parenchymal cells

Project description:We have established culture systems to generate liver progenitor cells (LPCs), liver sinusoidal endothelial cells (LSECs), and hepatic stellate cells (HSCs) from hiPSCs. Then, we established co-culture system of hiPSC-derived liver cells and performed RNA-seq of hiPSC-derived liver model.

2017-07-27 | GSE98710 | GEO

Single-Cell Analysis of Heterogeneity in Reverted Human iPSC-Derived Hepatic Stellate Cells

Project description:We employed a single-cell RNA sequencing (scRNA-seq) approach using the 10x Genomics platform to investigate the heterogeneity of reverted hepatic stellate cells (HSCs) derived from human induced pluripotent stem cells (hiPSCs). HSCs are key mediators of liver fibrosis; although their activation is well known to drive fibrogenesis, their fate following removal of the fibrogenic insult remains poorly understood. To address this, we utilized a hiPSC-derived liver culture system comprising hepatocytes, HSCs, and macrophages. HSCs were activated by hepatitis C virus (HCV) infection and subsequently treated with antivirals to achieve viral clearance. Reverted HSCs were then characterized through gene expression profiling, vitamin A quantification, functional assays, and scRNA-seq. Our data reveal that activated HSCs exhibit plasticity and can revert to a quiescent-like state upon removal of the activating stimulus, although they retain heightened sensitivity to re-stimulation. These findings enhance our understanding of HSC dynamics and highlight potential therapeutic strategies targeting HSC reversion to treat liver fibrosis.

2025-11-12 | GSE304675 | GEO

Expression profiling in hiPSC-derived liver sinusoidal endothelial cells and hepatic stellate cells

Project description:We have established culture systems to generate liver sinusoidal endothelial cells (LSECs) and hepatic stellate cells (HSCs) from hiPSCs. To characterize gene expression profiling in hiPSC-derived LSECs and HSCs, transcriptome analysis was performed.

2017-07-27 | GSE92771 | GEO

Transcriptome of human liver cells and culture-activated hepatic stellate cells

2016-03-02 | GSE68000 | GEO

RNA-sequence analysis of human iPSC-derived qHSCs, aHSCs and deactivated HSCs

Project description:Hepatic stellate cells (HSCs) are the major driver of fibrosis by expressing extracellular matrices (ECM). HSCs, in the normal liver, are quiescent and activated by liver injury to become myofibroblasts that proliferate and produce ECM. It has been shown that activated HSCs can be deactivated by removal of liver insults. In order to evaluate the changes of gene expression profiles, we induced quiescent HSCs (qHSCs), activated HSCs (aHSCs) and deactivated HSCs (daHSCs) from human induced pluripotent stem cells and performed RNA-sequence analysis.

2024-05-01 | GSE251824 | GEO

Expression data from HSCs

Project description:Hepatic stellate cells (HSCs) experience phenotypic transformation, from the quiescent phenotype to the activated one, after different etiologies of liver injury. Liver fibrosis is then occurred upon the activation of HSCs. miR-16 deficiency is identified to be an important characteristic of HSCs activation. We used Affymetrix rat 230 2.0 arrays (Affymetrix, Santa Clara, U.S.A.) to uncover the global alternations of transcriptome under miR-16 restoration. We isolated quiescent hepatic stellate cells (HSCs) from adult male SD rats (normal control group) by in situ perfusion and density-gradient centrifugation. Activated HSCs were separated from rats of fibrosis model group, which were treated by 40% carbon tetrachloride (CCl4) for 8 weeks, by means of liver section, digestion and sequential centrifugation. Quiescent and activated HSCs were then divided into 4 groups at random, namely quiescent HSCs, activated HSCs, pLV-miR-16-treated HSCs and pLV-GFP-treated HSCs. The pLV-miR-16-treated group, pLV-GFP-treated group were infected with pLV-miR-16 and pLV-GFP, respectively.

2012-08-28 | E-GEOD-40381 | biostudies-arrayexpress

Development of human iPSC-derived quiescent hepatic stellate cell-like cells for drug discovery and in vitro disease modeling

Project description:Hepatic stellate cells (HSCs) play a central role in the progression of liver fibrosis by producing extracellular matrices. The development of drugs to suppress liver fibrosis has been hampered by the lack of human quiescent HSCs (qHSCs) and an appropriate in vitro model that faithfully recapitulates HSC activation. In the present study, we developed a culture system to generate qHSC-like cells from human-induced pluripotent stem cells (hiPSCs) that can be converted into activated HSCs in culture. To monitor the activation process, a red fluorescent protein (RFP) gene was inserted in hiPSCs downstream of the activation marker gene actin alpha 2 smooth muscle (ACTA2). Using qHSC-like cells derived from RFP-reporter iPSCs, we screened a repurposing chemical library and identified therapeutic candidates that prevent liver fibrosis. Hence, hiPSC-derived qHSC-like cells will be a useful tool to study the mechanism of HSC activation and to identify therapeutic agents.

2021-12-02 | GSE155017 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data