Oxygenated perfusion enhances hepatocyte function in human iPSC-liver tissue

ABSTRACT: This study aimed to create functional and scalable tissue from hiPSC-derived liver organoids (hiPSC-LOs) by establishing an oxygenation system utilizing a decellularized liver (DL), which retains the parenchymal and vascular extracellular matrix to support cell adhesion and medium perfusion. An oxygenation system for hiPSC-LO-engrafted DLs (hiPSC-LT) was established using perfusion of oxygen-enriched medium containing artificial red blood cells. Perfusion with oxygenated medium containing artificial red blood cells suppressed cell death and promoted hepatic function of hiPSC-LTs by mimicking the physiological oxygen concentration found in the fetal liver. The oxygenation system using DLs and artificial red blood cells effectively supported the generation of transplantable, functional hiPSC-LT.

ORGANISM(S): Homo sapiens

PROVIDER: GSE314639 | GEO | 2026/06/23

REPOSITORIES: GEO