Transcriptomics

Dataset Information

0

Characterization of a novel N4 -methylcytosine restriction-modification system in Deinococcus radiodurans


ABSTRACT: Deinococcus radiodurans is an extremophilic microorganism that possesses a unique DNA damage repair system, conferring strong resistance to radiation, desiccation, oxidative stress, and chemical damage. Recently, we discovered that D. radiodurans possesses a N4-methylation (m4C) methyltransferase called M.DraR1, which recognizes the 5'-CCGCGG-3' sequence and methylates the second cytosine. Here, we revealed its cognate restriction endonuclease R.DraR1 and recognized that it is the only endonuclease specially for non-4C-methylated 5'-CCGCGG-3' sequence so far. We designated the particular m4C R.DraR1-M.DraR1 as DraI R-M system. Bioinformatics searches displayed the rarity of the DraI R-M homologues system. Meanwhile, recombination and transformation efficiency experiments demonstrated the important role of the DraI R-M system in response to oxidative stress. Besides, in vitro activity experiments showed that R.DraR1 could exceptionally cleave DNA substrates with m5C-methlated 5'-CCGCGG-3' sequence besides its routine activity, suggesting that this particular R-M component possesses a broader substrate choice. Furthermore, an imbalance of the DraI R-M system led to cell death through regulating genes involved in the maintenance of cell survival such as genome stability, transporter, energy production. Thus, our research revealed a novel m4C R-M system that plays key roles in maintaining cell viability and defending foreign DNA in D. radiodurans.

ORGANISM(S): Deinococcus radiodurans

PROVIDER: GSE251690 | GEO | 2024/02/14

REPOSITORIES: GEO

Similar Datasets

2022-07-10 | PXD028861 | Pride
2022-07-09 | PXD028866 | Pride
2014-07-07 | GSE59135 | GEO
| PRJNA513890 | ENA
2015-01-22 | GSE64952 | GEO
2021-09-09 | PXD020047 | Pride
2014-07-07 | E-GEOD-59135 | biostudies-arrayexpress
2015-01-22 | E-GEOD-64952 | biostudies-arrayexpress
2010-02-27 | GSE20383 | GEO
| PRJNA103493 | ENA