Project description:CO-culture of Microglia and Neurons. The NC/ APOE knocked down/ CD74 knocked down BV2 was stimulated by the ACP cystic fluid for 48 hours.
Project description:Direct conversion from fibroblast to neuron has recently been successfully induced bypassing the pluripotent state. However, the conversion takes a few months with low percentages of success. Here we found that depletion of p53, which can converted fibroblasts into three major neural lineages: neurons, astrocytes and oligodendrocytes. Furthermore, our method provided a high efficiency of conversion in aging fibroblasts, where published methods failed. This finding may help developing a prototype for neuron replacement therapy, including foraging people vulnerable to neurological disorders. p53 has been shown to inhibit reprogramming of fibroblasts to iPS cells, by depletion of p53 in human fibroblasts, we study the function of p53 in induced neuron process. By induction of p53 knockdown fibroblasts with special neuron medium, we can get mature neurons directly. In the induction process, many neurogenic transcription factors were up-regulated, and we prove that p21 is not involved in this process.
Project description:To investigate the effects of pathological IFN-γ stimulation on neurons in the context of subsequent activation, we established cell cultures of primary mouse neurons receiving pathological priming or no priming. We then performed RNA-Seq on these cells after vehicle, physiological, and pathological IFN-γ restimulation.
Project description:To investigate the possible roles on mitotic/microtubule-related genes during hippocampal neuron differentiation, we dissected E18.5 mouse embryonic hippocampi, cultured neurons in vitro and collected total RNA samples from different timepoints of differentiation.
Project description:To investigate the mode of action and potential side-effects we analyzed differential gene expression in
post-mitotic C9orf72 iPSC-Neurons by RNAseq
