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Effect of trmA on mRNA expression in Pseudomonas aeruginosa

ABSTRACT: tRNA modification involves in regulation of gene expression in bacterial response to environmental stresses. Here, we aimed to study the transcriptional profiling in the absence of trmA, a tRNA uracil-5-methyltransferase, compared to that of the wild type in Pseudomonas aeruginosa PA14 strain using Illumina sequencing. The disruption of trmA resulted in up and down regulated genes in many pathways based on gene ontology biological processes. One of these pathways was heme biosynthesis, in which the genes were down regulated in the trmA mutant. The finding was consistent with hypersensitivity towards hydrogen peroxide in the trmA mutant strain compared to that of the wild-type strain.

ORGANISM(S): Pseudomonas aeruginosa

PROVIDER: GSE252633 | GEO | 2025/06/08

REPOSITORIES: GEO

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Effect of trmA on tRNA expression in Pseudomonas aeruginosa

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