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Dynamic fibroblast-immune interactions shape recovery after brain injury [snRNASeq_Timecourse]

ABSTRACT: Fibroblasts and immune cells coordinate tissue regeneration and necessary scarring after injury. In the brain, fibroblasts are border-enriched cells whose dynamic molecular states and immune interactions after injury remain unclear. We used single nuclear RNA sequencing at multiple timepoints to profile stromal and immune responses to sterile (photothrombotic, PT) injury. We find that early pro-fibrotic myofibroblasts transition into several states, including meningeal and lymphocyte-interactive fibroblasts.

ORGANISM(S): Mus musculus

PROVIDER: GSE254162 | GEO | 2025/06/30

REPOSITORIES: GEO

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Dynamic fibroblast-immune interactions shape recovery after brain injury [Visium]

Project description:Fibroblasts and immune cells coordinate tissue regeneration and necessary scarring after injury. In the brain, fibroblasts are border-enriched cells whose dynamic molecular states and immune interactions after injury remain unclear. We used spatial transcriptomics (10X Visium) to spatiotemporally profile CNS wound healing responses, including stromal and immune responses, after photothrombotic (PT) injury.

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Dynamic fibroblast-immune interactions shape recovery after brain injury [snRNASeq_Cxcl12]

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Dynamic fibroblast-immune interactions shape recovery after brain injury [snRNASeq_Tgfb]

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Single-cell atlas reveals meningeal leukocyte heterogeneity in the developing mouse brain

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2021-07-23 | GSE173769 | GEO

Single-cell RNA-sequencing of CD45+ cells isolated from mouse dura mater or brain after photothrombotic injury

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JUN promotes hypertrophic skin scarring via CD36

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Characterization of meningeal type 2 innate lymphocytes and their response to CNS injury

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