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Serotonin and 5-HT5A Receptor Induce Melanogenic Lineage Differentiation and Regeneration

ABSTRACT: Serotonin and its receptors have been detected in oocytes and early embryos in various invertebrates and vertebrates and the serotonin pathway is involved in the regulation of vertebrate neural crest migration, affecting the cytoskeleton and modulating the vital activities of many cell types. Our previous studies have shown that serotonin promotes melanin synthesis in mouse melanoma cell lines. In addition, serotonin induces melanogenic lineage specification during early zebrafish embryos development. However, the exact molecular mechanism is unclear. We found that the number of melanocytes in zebrafish decreased significantly after htr5aa knockout, which mediated the development of melanocytes regulated by serotonin. Transcriptional analysis of zebrafish embryos showed that the htr5aa mutation had no effect on the expression of most neural crest specialized marker genes, but regulated the development of melanocytes by regulating the expression of pan-neural crest marker crestin. Furthermore, we found that HTR5A receptor mediates the regeneration of melanocytes, which provides a theoretical basis for the development of drugs to treat depigmentation diseases.

ORGANISM(S): Danio rerio

PROVIDER: GSE260502 | GEO | 2025/07/01

REPOSITORIES: GEO

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