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MOS4-Associated Complex subunits 3A and 3B modulate FLM splicing to repress floral transition in short day

ABSTRACT: Flowering initiation is a crucial and complex process tightly regulated at the molecular level. Alternative splicing introduces transcript variability under different environments. MAC3A and MAC3B, components of the MOS4-associated Complex (MAC) in Arabidopsis, play pivotal roles in this process. This study investigates the intricate role of MAC3A and MAC3B in shaping the flowering cascade via alternative splicing. Previous observations on the flowering phenotype of mac3a/mac3b knockdown mutants highlight the negative role of MAC3A and MAC3B on flowering initiation specifically under short-day conditions, but the mechanism remains unknown. To explore the underlying reason, We perform RNA-seq analysis, which reveals extensive splicing defects in flowering initiation pathways, indicating the broad influence of MAC3A and MAC3B on flowering induction. Moreover, mac3a/mac3b mutants exhibit the early flowering trait, most possibly because of the deficient FLOWERING LOCUS C (FLC) expression and the abnormal splicing patterns FLOWERING LOCUS M (FLM), namely the lower ratio between cool-temperature-induced FLM- to warm-temperature-induced FLM- , under short days.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE261591 | GEO | 2026/03/25

REPOSITORIES: GEO

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