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ScRNA-seq assessment of the impact of anti-IL6R and anti-OSM in a model of fibrotic lung disease during fibrosis (day 14) [NGS4272]

ABSTRACT: To determine at the single-cell level the transcriptional impact in the immune, stromal, endothelial and epithelial cell populations in the lung of IL-6 and/or OSM signaling in the bleomycin-induced model of lung injury and fibrosis (day 14).

ORGANISM(S): Mus musculus

PROVIDER: GSE263041 | GEO | 2026/01/06

REPOSITORIES: GEO

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scRNA-seq assessment of the impact of anti-IL6R and anti-OSM in a model of fibrotic lung disease during resolution (day 24) [NGS4659]

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scRNA-seq assessment of the impact of anti-IL6R and anti-OSM in a model of fibrotic lung disease during fibrosis (day 14) [NGS4272]

Project description:scRNA-seq assessment of the impact of anti-IL6R and anti-OSM in a model of fibrotic lung disease during fibrosis (day 14) [NGS4272]

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Assessment of OSMR dependency in normal human lung fibroblasts [NGS4564]

Project description:To investigate OSMR dependency in normal human lung fibroblasts, cells were preincubated with anti-OSM, or (one of 2) anti-OSMR, or anti-OSM+anti-OSMR antibodies, then stimulated with OSM or PBS for 24 hours.

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RNA-sequencing comparison of pneumonectomy and bleomycin reveals novel anti-fibrotic mechanisms and regulators

Project description:Fibroblast activation is transient in successful wound repair, but persistent in fibrotic pathologies. Understanding fibroblast deactivation during successful wound healing may provide new approaches to therapeutically reverse fibrogenic cell activation. To characterize the gene programs that accompany fibroblast activation and reversal during lung fibrosis resolution, we used RNA-seq analysis of flow-sorted Col1α1-GFP positive and CD45, CD31, and CD326 negative cells isolated from the lungs of young mice exposed to bleomycin and pneumonectomy. We compared fibroblasts isolated from controls to those isolated at days 14 and 30 after bleomycin exposure, as well as at 14 days after pneumonectomy, representing the peak of extracellular matrix deposition, early stage of fibrosis resolution, and non-fibrotic lung repair respectively.

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Oncostatin M (OSM) and Leukemia Inhibitory factor (LIF) treatment of osteoblasts via LIF and OSM receptors

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2014-12-13 | E-GEOD-53295 | biostudies-arrayexpress

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