Dataset Information


Expression data for Mobilized Peripheral Blood CD34+ cells with Wnt or BMP stimulation

ABSTRACT: Analysis of mobilized peripheral blood CD34+ cells from a healthy volunteer under erythroid differentiation conditions with and without stimulation to the BMP or Wnt signaling pathways. For erythroid differentiation, expanded CD34+ cells were placed in Stemspan SFEM medium supplemented with 2% pen/strep, 20ng/ml SCF, 1U/ml Epo, 5ng/ml IL3, 2uM dexamethasone, and 1uM beta-estradiol. Arrays were performed 2 hours after addition of cytokines. For signaling pathway stimulation, cells were exposed to 0.5uM BIO (a GSK3 inhibitor) for Wnt pathway activation, 25ng/ml rhBMP4 for BMP pathway activation, or vehicle control for 2 hours. Three biological replicates were performed per treatment group. We used microarrays to detail the global program of gene expression changes after Wnt or BMP pathway stimulation in human CD34+ hematopoietic progenitors under erythroid differentiation conditions. Overall design: To investigate the changes to gene expression in human CD34+ hematopoietic progenitors following stimulation of the Wnt or BMP pathways during the early stages of erythroid differentiation. Three biological replicates were performed per treatment group.

INSTRUMENT(S): [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array

ORGANISM(S): Homo sapiens  

SUBMITTER: Teresa Venezia Bowman 

PROVIDER: GSE26351 | GEO | 2011-10-07



Similar Datasets

2011-10-06 | E-GEOD-26351 | ArrayExpress
2012-05-31 | E-GEOD-37869 | ArrayExpress
| GSE26775 | GEO
2011-01-22 | E-GEOD-26775 | ArrayExpress
| GSE53263 | GEO
2011-10-06 | E-GEOD-29196 | ArrayExpress
2015-03-30 | E-GEOD-62517 | ArrayExpress
| GSE86910 | GEO
2019-11-23 | MSV000084614 | MassIVE
| GSE90940 | GEO