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Reciprocal Embryo-Endometrial adaptation at Implantation Onset Revealed by 3D Human Blastoid/Blastocyst-Endometrial Assembloid Co-Culture

ABSTRACT: We report that the three-dimensional (3D) maternal-fetal co-culture model using blastoid and endometrial assembloids captures the characteristics of embryo implantation and post-implantation development, including embryo attachment and expansion, trophoblasts differentiation, and gastrulation development. Importantly, we identify co-culture-induced trophoblast subsets marked by SDC1⁺MMP7⁺ and enriched for transcriptional features associated with extracellular matrix remodeling and cell migration. These subsets are reproducibly detected across independent implantation-like systems, emerge specifically within a decidualized stromal co-culture context, and are further linked to stromal-derived CSF3 signaling by intercellular communication analysis and targeted perturbation. In parallel, the endometrium displays transcriptional changes associated with enhanced decidualization and a glandular hypersecretory transition. Together, our study provides a tractable framework for dissecting reciprocal embryo-endometrial adaptation at the onset of implantation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE263528 | GEO | 2026/04/09

REPOSITORIES: GEO

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