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Epitranscripomic markers in microbiome-derived cell-free RNA from plasma reveal potential for colorectal cancer detection

ABSTRACT: Circulating cell-free RNA (cfRNA) in human plasma represents a potential new avenue for cancer detection. We report the Low-Input Multiple Methylation Sequencing (LIME-seq) to profile methylation patterns within cfRNA, detecting diverse tRNAs and small non-coding RNAs (ncRNAs) originating from both the human genome and microbiome. Unlike abundance profiles through cfRNA/cfDNA shaped by microbial turnover, we find that methylation patterns in microbiome-derived cfRNA accurately reflect microbiota activity within the host, offering a unique class of non-invasive biomarkers with highly promising early colorectal cancer (CRC) diagnosis potential.

ORGANISM(S): Homo sapiens Stenotrophomonas maltophilia

PROVIDER: GSE264208 | GEO | 2025/05/16

REPOSITORIES: GEO

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