Transcriptomic landscape of human stromal cells upon therapy-induced senescence and/or senotherapeutic intervention with melatonin

ABSTRACT: In eukaryotes, cellular senescence is a highly conserved stress response elicited by acute or chronic damage signals. In humans, senescent cells accumulate in multiple tissues at different rates, from 2- to 20-fold when comparing young (<35 years) to old (>65 years) healthy donors. The pathogenic role of cellular senescence in the vast majority of age-related diseases can be explained by the senescence-associated secretory phenotype (SASP). Melatonin is a methoxyindole synthesized and secreted mainly by the pineal gland during night under normal light/dark conditions. The endogenous rhythm of melatonin secretion is governed by the suprachiasmatic nuclei and entrained to the light/dark cycle. Light is able to either suppress or synchronize melatonin production in accordance with the light schedule. The nycthohemeral rhythm of this hormone can be evaluated by repeated measurement of plasma or saliva melatonin or urine sulfatoxymelatonin, the main hepatic metabolite. The primary physiological function of melatonin, whose secretion adjusts to night length, is to convey information concerning the daily cycle of light and darkness to body structures. However, there is a lack of insights into the biological capacity of melatonin in modulation of chronological aging and regulation of organismal healthspan. In this study, we aimed to evaluate the anti-aging potential of melatonin, by profiling the influence of melatonin on the transcriptome-wide expression of human stromal cells made senescent by genotoxicity in vitro. Together, this is the first report to determine the possibility of employing melatonin as a source of endogenous rhythm-related molecules in anti-aging pipelines, particularly pharmacological development for future geriatric medicine.

ORGANISM(S): Homo sapiens

PROVIDER: GSE264364 | GEO | 2025/12/31

REPOSITORIES: GEO