Transcriptomics

Dataset Information

25

The Adaptive Response of Endothelial Cells to Increased Shear Stress


ABSTRACT: In this study, we characterized the adaptive response of arterial endothelial cells to acute increases in shear stress magnitude in well-defined in vitro settings. Porcine endothelial cells were preconditioned by a basal level shear stress of 15 ± 15 dynes/cm2 at 1 Hz for 24 hours, and an acute increase in shear stress magnitude (30 ± 15 dynes/cm2) was then applied. The transcriptomics studies using microarray identified genes that were sensitive to the elevated shear magnitude. A significant number of the identified genes in our study are previously unknown as sensitive to shear stress. Overall design: Porcine endothelial cells were preconditioned by a basal level shear stress of 15 ± 15 dynes/cm2 at 1 Hz for 24 hours, and an acute increase in shear stress magnitude (30 ± 15 dynes/cm2) was then applied. Gene expression at multiple time points was measured using microarray.

INSTRUMENT(S): Duke Operon Porcine 10.5K Oligo Array

ORGANISM(S): Sus scrofa  

SUBMITTER: Ji Zhang  

PROVIDER: GSE26505 | GEO |

SECONDARY ACCESSION(S): PRJNA136681

REPOSITORIES: GEO

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