Reversal of Flow-Direction is A Critical Mechanical Stimulus for Full Activation of Endothelial Arteriogenesis Signaling Pathways

ABSTRACT: This study characterizes the response of primary human endothelial cells (human umbilical vein endothelial cells, HUVECs) to the relative shear stress changes that occur during the initiation of arteriogenesis at the entrance regions to a collateral artery network. HUVECs were preconditioned to a baseline level of unidirectional shear of 15 dynes/cm2 for 24 hours. After 24 hours preconditioning, HUVECs were subjected to an arteriogenic stimulus that mimics the shear stress changes observed in the opposing entrance regions into a collateral artery network. The arteriogenic stimulus consisted of a 100% step wise increase in shear stress magnitude to a unidirectional 30 dynes/cm2 in either the same or opposite direction of the preconditioned shear stress. This simulates either the feeding ent

ORGANISM(S): Homo sapiens

SUBMITTER: Stephen Turner 

PROVIDER: E-GEOD-46248 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress