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Transcriptome analysis of chemically polarised rat macrophages: in search of target genes for ARDS therapy

ABSTRACT: Acute respiratory distress syndrome (ARDS) is a severe clinical manifestation of an acute lung injury (ALI), which is characterized by poor oxygenation and non-compliant or "stiff" lungs. Modern guidelines for the treatment of ARDS include only symptomatic therapy: ventilator support, prone positioning, sedation and medications to prevent movement, diuretic medication for excess liquid removing, extracorporeal membrane oxygenation (ECMO). Lack of the pathogenic ARDS treatment contributed to the actualization of the search for new therapeutic approaches. Cell therapy is one of such approaches which are now of great interest in the ARDS therapy. Macrophages are known to play a key role in the pathogenesis of ARDS. Depending on the stage of the disease, macrophages exhibiting pro-inflammatory (M1) and anti-inflammatory (M2) states. It has been suggested that the utilisation of M2 macrophages in the early stages of the disease may contribute to a positive disease outcome. The aim of this study was to induce an anti-inflammatory phenotype of macrophages by knockdown of genes selected on the basis of transcriptome analysis of chemically polarised macrophages.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE266262 | GEO | 2025/04/30

REPOSITORIES: GEO

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