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Male autism spectrum disorder is linked to brain aromatase disruption by prenatal BPA in multimodal investigations and 10HDA ameliorates the related mouse phenotype (10HDA)

ABSTRACT: Primary cortical neurons were obtained from male Cyp19-EGFP mouse embryos at gestational day 15.5. Embryos were genotyped for SRY to determine sex, and only male embryos were used. Cells were seeded in 24-well plates containing 12 mm glass coverslips, coated with 100 µg/mL poly D-lysine to a density of ~0.45 x 106 cells/well and incubated in a humidified CO2 incubator (5% CO2, 37°C). Primary cortical from Cyp19-EGFP mouse embryos were obtained on GD 15.5. Neuronal cell cultures were treated with vehicle or 1mM 10HDA (Matreya, PA ,USA)

ORGANISM(S): Mus musculus

PROVIDER: GSE266400 | GEO | 2024/05/14

REPOSITORIES: GEO

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Male autism spectrum disorder is linked to brain aromatase disruption by prenatal BPA in multimodal investigations and 10HDA ameliorates the related mouse phenotype (BPA)

Project description:Pregannt Cyp19-EGFP mice (maintained on a soybean free mouse chow diet) were treated with either 50μg/kg/day Bsiphenol A or vehicle (ethinol in peanut oil) between gestational days (GD) 10.5-14.5. On GD15.5, fetuses were dissected and sex determined by SRY genotyping. Fetuses were obtained from different dams.

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Expression data from E2f7/E2f8/E2f3a null placentas and embryos

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2012-08-31 | E-GEOD-30488 | biostudies-arrayexpress

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Project description: Not available

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