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Transcriptomic changes in HT29 cells and a patient-derived CRC 3D model after CRISPRi-based KAT2A knockdown

ABSTRACT: We were interested in transcriptional changes after depletion of histone acetyltransferase KAT2A in human colorectal cancer. Therefore, we utilized HT29 cells and a patient-derived CRC 3D spheroid line (CRC3) as model, and analyzed global alterations of gene expression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE268709 | GEO | 2025/03/28

REPOSITORIES: GEO

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Transcriptomic changes in CRC1 and CRC2 patient-derived CRC 3D models after CRISPRi-based KAT2A knockdown

Project description:We were interested in transcriptional changes after depletion of histone acetyltransferase KAT2A in human colorectal cancer. Therefore, we utilized patient-derived CRC 3D spheroids CRC1 and CRC2 as models, and analyzed global alterations of gene expression.

2025-03-28 | GSE287741 | GEO

Transcriptomic changes in HCT116 cells after KAT2A knockout

Project description:We were interested in transcriptional changes after depletion of histone acetyltransferase KAT2A in human colorectal cancer. Therefore, we utilized HCT116 cells as model, and analyzed global alterations of gene expression.

2024-11-01 | GSE246881 | GEO

Transcriptomic changes in HT29 cells and a patient-derived CRC 3D model after CRISPRi-based KAT2A knockdown

Project description:Transcriptomic changes in HT29 cells and a patient-derived CRC 3D model after CRISPRi-based KAT2A knockdown

| PRJNA1118305 | ENA

Divergent Functions of Histone Acetyltransferase Paralogs KAT2A and KAT2B in Keratinocyte Stemness and Differentiation

Project description:The mammalian epidermis undergoes constant renewal replenished by a pool of stem cells and terminal differentiation of their progeny. This is accompanied by changes in gene expression and morphology orchestrated, in part, by epigenetic modifiers. Here, we defined the role of histone acetyltransferase KAT2A in epidermal homeostasis and provided a comparative analysis that revealed key functional divergence with its paralogue, KAT2B. In contrast to KAT2B's reported function in epidermal differentiation, KAT2A supports the undifferentiated state in keratinocytes. RNA-seq analysis of KAT2A- and KAT2B- depleted keratinocytes revealed dysregulated epidermal differentiation. Depletion of KAT2A led to premature expression of epidermal differentiation genes in the absence of inductive signals, whilst loss of KAT2B delayed differentiation. KAT2A acetyltransferase activity was indispensable in regulating epidermal differentiation gene expression. The metazoan-specific N-terminus of KAT2A was also required to support its function in keratinocytes. We further showed that the interplay between KAT2A- and KAT2B- mediated regulation was important for normal cutaneous wound healing in vivo. Overall, these findings reveal a distinct mechanism in which keratinocytes utilize a pair of highly homologous histone acetyltransferases to support divergent functions in self-renewal and differentiation processes.

2023-06-09 | GSE215244 | GEO

ACSS2-coupled KAT2A regulates histone H3 lactylation in gene promoter regions and gene expression

Project description:We report EGFR activation induces ERK-mediated S267 phosphorylation of acetyl-CoA synthetase 2 (ACSS2) and its subsequent nuclear translocation and complex formation with lysine acetyltransferase 2A (KAT2A).Consequently, KAT2A acts as a lactyltransferase to lactylate histone H3, leading to expression of Wnt/β-catenin, NFκB, and PD-L1, and brain tumor growth and immune evasion.

2024-10-01 | GSE278139 | GEO

Kat2a regulates hippocampal memory formation [RNA-seq]

Project description:We sequenced mRNA from 24 samples extracted from mouse CA1 tissue to generate the first CA1-specific murine transcriptome and the first CA1-transcriptome in response to environmental novelty under normal and Kat2a-loss-of-function conditions. Samples were divded in 4 groups: A: Control naM-CM-/ve (n=6), B: control novelty-exposed (n=5), C: Kat2a cKO naM-CM-/ve (n=6), D: Kat2a cKO novelty-exposed (n=7). Pairwise comparisons for AvsB, AvsC, BvsD and CvsD were performed using DESeq2.

2014-07-16 | E-GEOD-53380 | biostudies-arrayexpress

Kat2a

Project description:Rattus norvegicus Kat2a, lysine acetyltransferase 2A [Source:RGD Symbol;Acc:1307242], is differentially expressed in 24 experiment(s);

| ENSRNOG00000018364 | ExpressionAtlas

Kat2a

Project description:Rattus norvegicus Kat2a, lysine acetyltransferase 2A [Source:RGD Symbol;Acc:1307242], is expressed in 4 baseline experiment(s);

| ENSRNOG00000018364 | ExpressionAtlas

PALB2 acetylation by KAT2A/B (GCN5/PCAF) in vitro

Project description:PArtner and Localizer of BRCA2 (PALB2) is essential to maintain genome stability in human cells. Upon DNA damage by double DNA strand breaks, PALB2 is required to repair DNA by homologous recombination. In undamaged conditions, PALB2 protects coding regions, by preventing DNA stress due to collisions between transcription and replication machineries. PALB2 associates with chromatin, which is essential to fulfill its function in genome stability maintenance, however molecular mechanisms regulating PALB2 chromatin association remain unknown. In our previous published study describing the KAT2A/B(GCN5/PCAF)-acetylome (Fournier et al., Nat.Comm. 2016, doi: 10.1038/ncomms13227) we have identified PALB2 as an acetylated protein target of the acetyltransferases KAT2A (GCN5) and KAT2B (PCAF) in vivo. KAT2A/B-acetylated sites of PALB2 were mapped within its DNA/Chromatin association domain. In this current study, we have conducted in vitro acetyltransferase (AT) assays, by mixing purified recombinant GST-tagged PALB2 full-length (FL) and PALB2 fragment P2.2 which associates with DNA/chromatin (from residues 295-610), with Flag-PCAF, Flag-GCN5 and Flag-GCN5 catalytic mutant (mut), followed by proteomics analysis to map PALB2 acetylated lysines by KAT2A(GCN5) and KAT2B(PCAF) in vitro. PALB2 FL and P2.2 alone, or PALB2 FL and P2.2 mixed with GCN5 catalytic mutant, were used as negative controls.

2023-03-10 | PXD014681 | Pride

Kat2a regulates hippocampal memory formation [small RNA-seq]

Project description:We sequenced small RNAs from 12 samples extracted from mouse CA1 tissue to generate the first CA1-specific murine miRNome under normal and Kat2a-loss-of-function conditions. Samples were divded in 4 groups: A: Control (n=6), C: Kat2a cKO naÃ¯ve (n=6)

2014-07-16 | E-GEOD-53427 | biostudies-arrayexpress

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