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A core pattern-induced transcribed enhancer regulates the immune response to fungi in Arabidopsis by regulating the synthesis of camalexin

ABSTRACT: Effective plant defense against pathogens relies on highly coordinated regulation of immune gene expression. Enhancers, as cis-regulatory elements, are indispensable determinants of the dynamic gene regulation, but the molecular functions in plant immunity are not well understood. In this study, we identified a novel enhancer, CORE PATTERN-INDUCED ENHANCER 35 (CPIE35), is rapidly activated upon pathogenic elicitation and negatively regulates camalexin synthesis and anti-fungal resistance through modulating WRKY15 expression. During immune activation, CPIE35 activates the transcription of WRKY15 by forming chromatin loops with the promoter of WRKY15 in a WRKY18/40/60-, WRKY33-, and MYC2-dependent manner. WRKY15 directly binds to the promoters of GSTU4 and PAD3 and suppresses their expression, leading to the reduced camalexin synthesis and resistance. Interestingly, the CPIE35 region is evolutionally conserved among distantly related dicots, and the CPIE35-WRKY15 module exerts similar functions in Brassica napus to negatively regulates antifungal resistance. Our work reveals the “TFs-enhancer-promoter” regulatory mechanism in maintenance of immune hemostasis and highlights the importance and conservation of enhancers in fine-tuning immune gene expression in plants.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE271462 | GEO | 2025/01/28

REPOSITORIES: GEO

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