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Znf706 regulates germplasm formation and primordial germ cell development in zebrafish

ABSTRACT: The cell fate of primordial germ cells (PGCs) in zebrafish is pre-determined by maternally deposited germplasm, which is packaged into ribonucleoprotein complex in oocytes and, after fertilization, inherited into PGCs promoting germline fate in embryos. However, the maternal factors regulating the assembly of germplasm and PGC development remain poorly understood. In this study, we report that the maternal transcription factor Znf706 regulates the assembly of germplasm factors into a granule-like structure localized perinuclearly in PGCs during migration. Maternal and zygotic mutants of znf706 (MZznf706) exhibited deficient germplasm at the early embryonic stage, decreased PGC numbers with ectopic PGC locations during PGC migration, and lower female ratio in adulthood. Notably, the implementation of Znf706 CUT&Tag and RNA-seq on immature oocytes uncovered that Znf706 in stage I oocytes may act as a transcriptional activator and mediate mRNA binding, translational regulation, and metabolic pathways of oocytes. Hence, we propose that Znf706 is crucial for germplasm assembly and PGC development in zebrafish.

ORGANISM(S): Danio rerio

PROVIDER: GSE272199 | GEO | 2025/07/28

REPOSITORIES: GEO

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