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Transcriptomic profiling of day 194 patient-specific pluripotent stem cell-derived alveolar epithelial type 2 cells expressing the SFTPCC121Y mutation and their gene-edited corrected counterparts

ABSTRACT: We performed transcriptomic profiling of cells derived from human induced pluripotent stem cells (iPSCs) using our previously described distal lung directed differentiation protocol to generate alveolar epithelial type 2 cells (iAT2s). We used the SPC8 human iPSC line and specifically the SPC8-ST-Corr (SFTPCtdT/WT) and SPC8-ST-Mut (SFTPCC121Y/tdT) clones containing a SFTPCtdTomato knock-in reporter. SFTPCtdTomato+ cells were sorted on days 45, 87 and 182 of differentiation. iAT2s were single-cell passaged in self-renewing 3D alveolosphere cultures approximately every 10-14 days through day 194. On day 194, all live cells were sorted and encapsulated for scRNA-seq using the 10X Chromium System. We find that a subset of mutant (SFTPCC121Y/tdT) iAT2s develops an aberrant transitional cell state.

ORGANISM(S): Homo sapiens

PROVIDER: GSE272862 | GEO | 2025/07/25

REPOSITORIES: GEO

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