Identification of novel genetic interactions with Recql4 in a genome-wide CRISPR/Cas9 synthetic lethal screen.

ABSTRACT: This study was performed to provide a detailed understanding of the functions and pathways that intersect with the essential DNA replication factor RECQL4. Mutations in RECQL4 cause Rothmund-Thomson Syndrome (RTS), a familial cancer syndrome associated with early onset osteosarcoma in a high proportion of patients. Using murine RECQL4 mutations that closely map to recurrent human RTS mutations, we performed an unbiased genome-wide screen to identify genes that, when deleted, rescued the phenotypes associated with RECQL4 mutation.

ORGANISM(S): Mus musculus

PROVIDER: GSE273006 | GEO | 2025/11/27

REPOSITORIES: GEO