Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Immediate and delayed hypothalamic response to monochromatic green illumination during the avian embryonic period are mediated thorough epigenetic modulations

ABSTRACT: In this study, we investigated the effects of light-induced fetal responses in avian species, specifically focusing on green monochromatic illumination (GMI). Broiler eggs were exposed to GMI either throughout the entire incubation period (Green) or specifically during the final three days before hatching (G3D), with control groups incubated in dark or white light conditions. Unbiased genome-wide analyses at day of hatch (DOH) revealed significant transcriptional changes in the hypothalamus of the G3D group, highlighting over 500 differentially expressed genes. In parallel with these transcriptional changes, our epigenetic analysis revealed that GMI exposure in the G3D group resulted in increased binding of phosphorylated CREB1 (pCREB1) and elevated levels of Histone H3 Lysine 27 acetylation (H3K27ac) at gene promoters linked to growth, appetite, and metabolism. Mechanistically, we found that pre-exposure to blue light, which disrupts green photoreceptor activity, reduced retinal green opsin levels and nullified the epigenetic modifications typically induced by GMI in the G3D group. Phenotypically, the G3D group exhibited increased post-hatch body weight at several stages, suggesting enhanced growth plasticity and metabolic efficiency, though these effects diminished with age. This study underscores the significant role of specific wavelengths and timing of light exposure in shaping developmental trajectories, and highlight the crucial role of epigenetics in shaping organismal responses to their environment.

ORGANISM(S): Gallus gallus

PROVIDER: GSE273328 | GEO | 2025/07/16

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

The immediate effect of high-intensity green LED stimulation on the gene expression profile of S. coelicolor A3(2)

Project description:We investigated a novel, simple approach to induce the production of cryptic secondary metabolites in actinomycetes by stimulating the organism with high-intensity monochromatic green light (180 radiation unit). Streptomyces coelicolor A3(2) produces blue antibiotic actinorhodin (ACT) and red antibiotic undecylprodigiosin (RED). Using these two pigment antibiotics as indicators, we found that sporulation acceleration and regulation of the antibiotic production pathways can be induced by using high-intensity monochromatic green LEDs. Therefore, we investigated the immediate response of S. coelicolor A3(2) gene expression to the strong green LED stimulation.

2023-12-10 | E-MTAB-12876 | biostudies-arrayexpress

Immediate and delayed hypothalamic response to monochromatic green illumination during the avian embryonic period are mediated thorough epigenetic modulations

Project description:Immediate and delayed hypothalamic response to monochromatic green illumination during the avian embryonic period are mediated thorough epigenetic modulations

| PRJNA1141430 | ENA

Transcriptome sequencing of rabbit cornea after different illumination

Project description:This study aimed to investigate the effects of long-term pollution from different wavelengths of light on the corneal epithelium (CE) and identify potential biomarkers. Rabbits were exposed to red, green, blue, white, and environmental light for 6 weeks. The CE was assessed using various techniques such as fluorescein sodium staining, transcriptome sequencing, electron microscopy, and molecular assays. In human corneal epithelial cells (hCECs), the downregulation of vascular cell adhesion molecular 1 (VCAM1) in response to blue light (BL) pollution was observed. This downregulation of VCAM1 inhibited hCEC migration and increased reactive oxygen species (ROS) levels, apoptosis, and inhibited the AKT/p70S6K cascade. Animal experiments confirmed that BL pollution caused similar effects on the rabbit cornea, including increased ROS production, apoptosis, delayed wound healing, and decreased VCAM1 expression. In conclusion, BL-induced VCAM1 downregulation may impair CE and wound healing, and promotes ROS and apoptosis in vitro and in vivo.

2023-11-18 | GSE242331 | GEO

BBX32 Role in Early Light Signaling in Arabidopsis

Project description:The transcriptional profiles of several genotypes of Arabidopsis seedling shoots were compared in dark and after 1 hr of monochromatic red-light treatment. The transcriptional profiles of several genotypes of Arabidopsis seedling shoots were compared in dark and after 1 hr of monochromatic red-light treatment.

2011-06-30 | E-GEOD-21174 | biostudies-arrayexpress

BBX32 Role in Early Light Signaling in Arabidopsis

Project description:The transcriptional profiles of several genotypes of Arabidopsis seedling shoots were compared in dark and after 1 hr of monochromatic red-light treatment.

2011-06-30 | GSE21174 | GEO

Effect of incubation in red, blue, and green monochromatic lights on gene expression in a species of Ostreococcus; RCC809

Project description:To investigate responses to light quality in this eukaryotic phytoplankton species, replicates were incubated for 72 hours in monochromatic light at 4umol photons m-2 s-1. We then performed gene expression profiling analysis using data obtained from RNA-seq of biological replicates in three light quality conditions.

2023-11-29 | GSE221420 | GEO

Light regulation of chlorophyll and glycoalkaloid biosynthesis during tuber greening of potato S. tuberosum

Project description:Potato, S. tuberosum, is one of the most important global crops, but has high levels of waste due to tuber greening under light, which is associated with the accumulation of neurotoxic glycoalkaloids. Here, we have investigated the effect of monochromatic far-red, red, and blue light on the regulation of chlorophyll and glycoalkaloid accumulation in tubers of a commercial variety, King Edward. Transcriptomic analysis of tubers exposed to red, blue, and white light showed that light induction of photosynthesis and tetrapyrrole-related genes grouped into two distinct patterns with one group showing much stronger induction in blue at 6 h and 24 h and a second group showing only red induction at 24 h.

2020-02-25 | E-MTAB-7707 | biostudies-arrayexpress

Molecular responses to prolonged darkness and subsequent acclimation to re-illumination in the diatom Phaeodactylum tricornutum

Project description:We have investigated both the response to prolonged darkness and the re-acclimation to M-bM-^@M-^\moderate intensityM-bM-^@M-^] white irradiance (E = 100 M-BM-5mol m-2 s-1) in the diatom Phaeodactylum tricornutum, using an integrated approach involving global transcriptional profiling, pigment analyses, imaging and photo-physiological measurements. The responses were studied during continuous white light, after 48 h of dark treatment and after 0.5 h, 6 h, and 24 h of re-exposure to the initial irradiance. The cultures were incubated at 15C under cool white fluorescent light at a scalar irradiance (EPAR) of ~100 M-BM-5mol m-2 s-1 under continuous white light (CWL) conditions. The cultures were dark-treated for 48 h (DT) before re-exposure to the previous light conditions for 0.5 h, 6 h or 24 h (WL 0.5, 6 and 24 h). Sampling of CWL cultures and DT cultures were performed in addition to the sampling at the WL incubation times of 0.5 h, 6 h, and 24 h. Three biological replicas for each of the five treatments were harvested.

2013-04-01 | E-GEOD-42039 | biostudies-arrayexpress

Proteomic Analysis of ARPE-19 Cells

Project description:We performed proteomic analysis of ARPE-19 cells in control group, bule light exposure group, blue light exposure with minocycline treatment group.

2021-12-14 | PXD030376 |

RNA-SEQ Reveals Changes in the Staphylococcus aureus Transcriptome following blue light illumination

Project description:Purpose: In an effort to better understand the mechanism of blue light inhibition in Staphylococcus aureus, a whole transcriptome analysis of S. aureus isolate BUSA2288 was performed using RNA-seq to analyze the differential gene expression in response to blue light exposure. Methods: RNA was extracted from S. aureus cultures pooled from 24 one ml well samples illuminated with a dose of 250 J/cm2 of 465 nm blue light, and from control cultures grown in the dark. Complementary DNA (cDNA) were generated from the RNA samples and then sequenced using the Illumina MiSeq Next Generation Sequencer. The combined results of 2 independent experiments were analyzed using the Pairwise Analysis tools in GeneSifter®. The genes were normalized by Mapped Reads using EdgeR statistics including a Benjamini and Hochberg false discovery rate correction. Quality was set at a minimum number of 10 reads and a p value of 0.05. The lower threshold for change was 5 fold. Results: Transcriptomic comparisons using a cutoff of 5 fold identified a total of 28 down-regulated genes and 6 up-regulated genes in the samples that were exposed to blue light. All but 6 of the differentially regulated genes fall into 8 functional categories: amino acid biosynthesis, cell envelope components, cellular processes, central intermediary metabolism, energy metabolism, protein synthesis, regulatory function, and transport and binding proteins. Five genes encode conserved proteins of unknown function. Conclusions: Blue light has been shown to be bactericidal against S. aureus and is a potential alternative therapy for antibiotic resistant organisms. The mechanism for the inactivation of bacteria is hypothesized to involve ROS. We have found evidence that supports this hypothesis that involves multiple pathways including essential metabolic pathways and known virulence pathways.

2015-10-04 | GSE62055 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data