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Effects of TMEM55B deficiency on hepatic gene expression of Western diet-fed male mice

ABSTRACT: Transmembrane protein 55B (TMEM55B) is a lysosomal protein that modulates lysosome position and function. We examined the impact of TMEM55B deficiency on mitochondrial function and the development of Metabolic Dysfunction Associated with Steatohepatitis (MASH). We found that several genes implicated in mitochondria function including mitophagy regulator Prkn (p=1.11e-10), and Immp2i (p=1.29e-22) and Sugct (p=8.82e-10) were downregulated in the livers of male mice treated with Tmem55b ASO compared to NTC ASO. In additon, the RNAseq analysis of 4-week Tmem55b vs. NTC ASO treatment demonstrated a functional enrichment of upregulated genes involved in cell adhesion (Flrt2, Egfl7, Col8a1, Pkp3, Pcdh17, Mcam) in the liver. Taken together, our data suggested that loss of TMEM55B accelerates diet-induced MASLD onset and progression in mice through impaired mitochondrial function.

ORGANISM(S): Mus musculus

PROVIDER: GSE273884 | GEO | 2026/01/14

REPOSITORIES: GEO

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