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TICRR/TRESLIN and MTBP Cut&Run in HCT116 cells with inhibited origin licensing

ABSTRACT: The selection of replication origins plays a crucial role in eukaryotic DNA replication. However, the mechanisms of origin selection in humans still need to be defined. Recent work in yeast has shown that the recruitment of Sld3-Sld7 to origins during G1 is involved in origin selection. In humans, TICRR/TRESLIN and MTBP are the orthologs of Sld3 and Sld7, respectively. In this study, we have mapped the genomic binding locations of TICRR/TRESLIN and MTBP in cells where loading of MCM2-7 complex has been inhibited by dox-inducible expression of a degradation-resistant Geminin(T25D).

ORGANISM(S): Homo sapiens

PROVIDER: GSE274465 | GEO | 2025/05/27

REPOSITORIES: GEO

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TICRR/TRESLIN and MTBP Cut&Run in Asynchronously Cycling HCT116 Cells

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TICRR/TRESLIN and MTBP Cut&Run in HCT116 cells with inhibited origin licensing

Project description:TICRR/TRESLIN and MTBP Cut&Run in HCT116 cells with inhibited origin licensing

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TICRR/TRESLIN and MTBP Cut&Run in Asynchronously Cycling HCT116 Cells

Project description:TICRR/TRESLIN and MTBP Cut&Run in Asynchronously Cycling HCT116 Cells

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Binding of the Treslin-MTBP Complex to Specific Regions of the Human Genome Promotes the Initiation of DNA Replication

Project description:In this study, we have mapped the binding sites for the MTBP subunit of the Treslin-MTBP complex throughout the human genome by using the CUT&RUN method. Our results have indicated that MTBP associates reproducibly with several tens of thousands of sites in the genome. Many binding sites contain either G4 DNA or an AP-1 motif in a nucleosome-free region at transcription start sites (TSSs) or enhancers, respectively. In addition, this nucleosome-free region is adjacent to a nucleosome containing certain histone marks characteristic of open chromatin that have also been implicated in initiation. We also mapped early initiation zones with EdU-labeling (EdU-seq) and found that these initiation domains contain a large number of MTBP binding sites. Taken together, these findings indicate that Treslin-MTBP associates with multiple genomic signals to promote initiation of replication.

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TICRR/TRESLIN and MTBP Cut&Run in G1 synchronized HCT 116 cells

Project description:TICRR/TRESLIN and MTBP Cut&Run in G1 synchronized HCT 116 cells

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