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TICRR/TRESLIN and MTBP Cut&Run in HCT116 cells with inhibited origin licensing

ABSTRACT: TICRR/TRESLIN and MTBP Cut&Run in HCT116 cells with inhibited origin licensing

PROVIDER: PRJNA1146464 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30192615_1.fastq.gz	Fastqsanger.gz
	SRR30192615_2.fastq.gz	Fastqsanger.gz
	SRR30192616_1.fastq.gz	Fastqsanger.gz
	SRR30192616_2.fastq.gz	Fastqsanger.gz
	SRR30192617_1.fastq.gz	Fastqsanger.gz

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TICRR/TRESLIN and MTBP Cut&Run in HCT116 cells with inhibited origin licensing

Project description:The selection of replication origins plays a crucial role in eukaryotic DNA replication. However, the mechanisms of origin selection in humans still need to be defined. Recent work in yeast has shown that the recruitment of Sld3-Sld7 to origins during G1 is involved in origin selection. In humans, TICRR/TRESLIN and MTBP are the orthologs of Sld3 and Sld7, respectively. In this study, we have mapped the genomic binding locations of TICRR/TRESLIN and MTBP in cells where loading of MCM2-7 complex has been inhibited by dox-inducible expression of a degradation-resistant Geminin(T25D).

2025-05-27 | GSE274465 | GEO

TICRR/TRESLIN and MTBP Cut&Run in Asynchronously Cycling HCT116 Cells

Project description:The selection of replication origins plays a crucial role in eukaryotic DNA replication. However, the mechanisms of origin selection in humans are not yet well-defined. Recent work in yeast has shown that the recruitment of Sld3-Sld7 to origins during G1 is involved in origin selection. In humans, TICRR/TRESLIN and MTBP are the orthologs of Sld3 and Sld7, respectively. In this study, we have mapped the genomic binding locations of TICRR/TRESLIN and MTBP in asynchronous cells.

2025-05-27 | GSE274463 | GEO

The TRESLIN-MTBP complex couples completion of DNA replication with S/G2 transition

Project description:It has been proposed that ATR kinase senses the completion of DNA replication to initiate the S/G2 transition. In contrast to this model, we show here that the TRESLIN-MTBP complex prevents a premature entry into G2 from early S-phase independently of ATR/CHK1 kinases. TRESLIN-MTBP acts transiently at pre-replication complexes (preRCs) to initiate origin firing and is released after the subsequent recruitment of CDC45. This dynamic behavior of TRESLIN-MTBP implements a monitoring system that checks the activation of replication forks and senses the rate of origin firing to prevent the entry into G2. This system detects the decline in the number of origins of replication that naturally occurs in very late S, which is the signature that cells use to determine the completion of DNA replication and permit the S/G2 transition. Our work introduces TRESLIN-MTBP as a key player in cell cycle control independent of canonical checkpoints.

2022-08-06 | GSE210129 | GEO

TICRR/TRESLIN and MTBP Cut&Run in G1 synchronized HCT 116 cells

2025-05-27 | GSE274464 | GEO

The TRESLIN-MTBP complex couples completion of DNA replication with S/G2 transition

2023-03-11 | PXD035353 | Pride

TICRR/TRESLIN and MTBP Cut&Run in Asynchronously Cycling HCT116 Cells

Project description:TICRR/TRESLIN and MTBP Cut&Run in Asynchronously Cycling HCT116 Cells

| PRJNA1146460 | ENA

Binding of the Treslin-MTBP Complex to Specific Regions of the Human Genome Promotes the Initiation of DNA Replication

Project description:In this study, we have mapped the binding sites for the MTBP subunit of the Treslin-MTBP complex throughout the human genome by using the CUT&RUN method. Our results have indicated that MTBP associates reproducibly with several tens of thousands of sites in the genome. Many binding sites contain either G4 DNA or an AP-1 motif in a nucleosome-free region at transcription start sites (TSSs) or enhancers, respectively. In addition, this nucleosome-free region is adjacent to a nucleosome containing certain histone marks characteristic of open chromatin that have also been implicated in initiation. We also mapped early initiation zones with EdU-labeling (EdU-seq) and found that these initiation domains contain a large number of MTBP binding sites. Taken together, these findings indicate that Treslin-MTBP associates with multiple genomic signals to promote initiation of replication.

2020-09-22 | GSE143481 | GEO

TICRR/TRESLIN and MTBP Cut&Run in G1 synchronized HCT 116 cells

Project description:TICRR/TRESLIN and MTBP Cut&Run in G1 synchronized HCT 116 cells

| PRJNA1146462 | ENA

The TRESLIN-MTBP complex couples completion of DNA replication with S/G2 transition

Project description:The TRESLIN-MTBP complex couples completion of DNA replication with S/G2 transition

| PRJNA863859 | ENA

The role of DDK and Treslin-MTBP in coordinating replication licensing and pre-initiation complex formation.

Project description:Treslin/Ticrr is required for the initiation of DNA replication and binds to MTBP (Mdm2 Binding Protein). Here, we show that in Xenopus egg extract, MTBP forms an elongated tetramer with Treslin containing two molecules of each protein. Immunodepletion and add-back experiments show that Treslin-MTBP is rate limiting for replication initiation. It is recruited onto chromatin before S phase starts and recruitment continues during S phase. We show that DDK activity both increases and strengthens the interaction of Treslin-MTBP with licensed chromatin. We also show that DDK activity cooperates with CDK activity to drive the interaction of Treslin-MTBP with TopBP1 which is a regulated crucial step in pre-initiation complex formation. These results suggest how DDK works together with CDKs to regulate Treslin-MTBP and plays a crucial in selecting which origins will undergo initiation.

| S-EPMC8548084 | biostudies-literature

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