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Transcriptomic changes in primary CD4+ T cells upon infection with HIV-1 harboring an intact or defective vpr open-reading frame

ABSTRACT: We performed unbiased RNA sequencing (RNA-seq) analyses of primary CD4+ T cells infected with HIV-1 harboring an intact or defective Vpr open reading frame and analyzed which genes are deregulated in a Vpr-dependent manner. Our transcriptome analyses showed that NFAT controls 46.5% of 1083 Vpr-deregulated genes in primary CD4+ T cells. Gene set enrichment analyses revealed that Vpr upregulated processes related to T cell signaling, proliferation, and immune function, while downregulating cell cycle progression, ribosome activity, and cytoskeleton organization. qRT-PCR confirmed Vpr-mediated modulation of specific genes, including the upregulation of NEIL1, TNFS4 and CXCL10, and the downmodulation of CCNB1, CDC20, CENPA and PLK1. Hence, a significant portion of Vpr-deregulated genes in primary CD4+ T cells are controlled by NFAT, affecting pathways related to T cell activation, cell cycle progression, cytoskeleton, and chromosome organization.

ORGANISM(S): Homo sapiens

PROVIDER: GSE274705 | GEO | 2026/01/29

REPOSITORIES: GEO

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Transcriptomic changes in primary CD4+ T cells upon infection with HIV-1 harboring an intact or defective vpr open-reading frame

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