Genomics

Dataset Information

0

Effect of sexual recombination on population diversity in aflatoxin production by Aspergillus flavus and evidence for cryptic heterokaryosis


ABSTRACT: Aspergillus flavus is the major producer of carcinogenic aflatoxins in crops worldwide. Natural populations of A. flavus show tremendous variation in aflatoxin production some of which can be attributed to extreme environmental conditions (e.g., drought), differential regulation of the aflatoxin biosynthetic pathway, missing cluster genes or loss-of-function mutations. Understanding the evolutionary processes that generate genetic diversity in A. flavus may also explain quantitative and qualitative differences in aflatoxigenicity. Several population studies provide indirect evidence of recombination in the aflatoxin gene cluster and genome-wide, using multilocus genealogical approaches. More recently A. flavus has been shown to be functionally heterothallic and capable of sexual reproduction in laboratory crosses. In the present study, we characterize the progeny from nine A. flavus crosses and show that crossovers in the aflatoxin cluster coincide with inferred recombination blocks and hotspots in natural populations, which suggests that recombination in the cluster is primarily driven by sex. Moreover, we show that a single crossover event in the cluster can restore aflatoxigenicity, which is significant as mycotoxin production in A. flavus is highly heritable. aCGH was used to corroborate inferences from cluster-based MLSTs and to possibly identify additional crosovers within the cluster.

ORGANISM(S): Zea mays Aspergillus oryzae Aspergillus flavus

PROVIDER: GSE27484 | GEO | 2012/03/07

SECONDARY ACCESSION(S): PRJNA138593

REPOSITORIES: GEO

Similar Datasets

| E-GEOD-27484 | biostudies-arrayexpress
2015-10-01 | GSE61981 | GEO
2008-06-18 | GSE8185 | GEO
| E-GEOD-8185 | biostudies-arrayexpress
2007-06-07 | GSE6182 | GEO
2013-10-22 | GSE51469 | GEO
2022-02-17 | PXD027517 | Pride
2018-02-27 | PXD007164 | Pride
2022-07-13 | PXD030015 | Pride
2022-06-06 | PXD030912 | Pride