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Study on transcriptome changes of placenta in mice after subchronic exposure to polystyrene nanoplastics

ABSTRACT: Nanoplastics, as an emerging persistent pollutant of global concern in recent years, have posed a potential threat to human health. However, there is little known about the adverse effects of nanoplastics on the female reproductive system. Here, polystyrene nanoplastics (PS-NPs) with a diameter of 50 nm were selected as representative nanosized plastic particles to investigate the potential effects of subchronic exposure on placenta development in mice. Maternal exposed to 10 mg/L PS-NPs via drinking water induced a significant increase in fetal weight. Further observation of the placental morphology showed that PS-NPs exposure caused aberrant placental structure and damaged trophoblast cells. At the cellular level, exposure to PS-NPs promoted the proliferation, migration and invasion ability of HTR-8/SVneo. Mechanistically, transcriptome and proteomic analysis reveal that PS-NPs triggered placental calcium disturbance and noticeably upregulated the STAM2 expression in mice. Importantly, STAM2 induced by PS-NPs mediates the imbalance of trophoblastic calcium homeostasis and regulates cell functions through disturbing calcium channel protein IP3R3 lysosome degradation in HTR-8/SVneo cells. Our results indicate that trophoblastic calcium dyshomeostasis is the main cause of abnormal placental development induced by PS-NPs subchronic exposure. These findings contribute to estimating the risk for PS-NPs on female reproductive and indicate a basis for prevention and treatment of placental impairment.

ORGANISM(S): Mus musculus

PROVIDER: GSE276033 | GEO | 2026/09/30

REPOSITORIES: GEO

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