Transcriptomics

Dataset Information

34

The intestinal stem cell signature identifies colorectal cancer stem cells and predicts disease relapse


ABSTRACT: Using EphB2 or the ISC marker Lgr5, we have FACS-purified and profiled intestinal stem cells (ISCs), crypt proliferative progenitors and late transient amplifying cells to define a gene expression program specific for normal ISCs. A frequent complication in colorectal cancer (CRC) is regeneration of the tumor after therapy. The intestinal stem cell signature predicts disease relapse in CRC and identifies a stem cell-like population that displays robust tumor- initiating capacity in immunodeficient mice as well as long-term self-renewal potential. Overall design: We FACS purified mouse intestinal crypt cells according to their EphB2 or Lgr5 contents. We used Affymetrix chips to hybridize 2 samples from EphB2 high, 2 samples from EphB2 medium and 2 samples from EphB2 low cells (one sample from each group in a first hybridization on February 2009 plus an additional sample from each group on March 2009). Additionally, we hybridized one sample from Lgr5-EGFP high and one sample from Lgr5-EGFP low cells, obtained from Lgr5-EGFP knock-in mice (Barker et al., 2007).

INSTRUMENT(S): [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array

ORGANISM(S): Mus musculus  

SUBMITTER: Eduard Batlle  

PROVIDER: GSE27605 | GEO | 2011-03-14

SECONDARY ACCESSION(S): PRJNA138281

REPOSITORIES: GEO

Similar Datasets

2011-03-14 | E-GEOD-27605 | ArrayExpress
2015-08-25 | E-GEOD-58532 | ArrayExpress
2015-08-25 | E-GEOD-64054 | ArrayExpress
2016-08-19 | E-GEOD-72190 | ArrayExpress
2012-08-15 | E-GEOD-36497 | ArrayExpress
2015-05-07 | E-GEOD-65253 | ArrayExpress
| GSE90856 | GEO
2013-04-19 | E-GEOD-46200 | ArrayExpress
| GSE123051 | GEO
2009-03-13 | GSE14201 | GEO