Transcriptomics

Dataset Information

49

Differential expression of microRNA profiles from the peripheral blood of type 1 and type 2 diabetic patients


ABSTRACT: Using microarray, we compared the expression of miRNAs from the peripheral blood of male subjects with T1DM and T2DM with healthy controls. Healthy male controls used were age-matched to the T2DM group patients with mean(SD) 37.3 (7.1) years. Subjects with T1DM were younger [23.3(1.6) years]. Expression was compared and validated using quantitative real-time PCR. Statistical testing (ANOVA, P-value <0.05) was performed and fold changes with respect to the control were calculated Systolic BP, fasting glucose, HbA1c, total cholesterol and LDL-cholesterol levels were significantly higher in T2DM subjects compared with controls (P-value <0.05). Compared with controls, we identified 37 differentially regulated miRNAs in DM subjects. Among them, 21 miRNAs were upregulated (2-5 fold change, p-value < 0.05) and 16 miRNAs were downregulated (1.5-2 fold change, p-value < 0.05). These miRNAs had gene putative targets primarily involved in regulating pancreatic development and functions, adipocyte differentiation, insulin signaling and glucose-dependent insulin secretion. Overall design: peripheral blood of male subjects with T1DM and T2DM with healthy controls

INSTRUMENT(S): miRCURY LNA microRNA Array, v.11.0 - hsa, mmu & rno [probe-level]

ORGANISM(S): Homo sapiens  

SUBMITTER: Kandiah Jeyaseelan  

PROVIDER: GSE27645 | GEO | 2011-03-05

SECONDARY ACCESSION(S): PRJNA138523

REPOSITORIES: GEO

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