Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of m6A on neuronal mRNA translocation from soma to neurites in the developing brain

ABSTRACT: Neurons are highly polarized cells with extensive neuronal processes, including dendrites and axons. All the RNA required by neurons is synthesized in the soma, while distinct neurites still require various RNAs. The soma and neurites contain different transcriptomes tailored to their specialized functions. m6A modification, the most abundant RNA modification in eukaryotes, can affect RNA stability and translation efficiency. Here, we suggest that m6A also affects RNA translocation in neurons and can alter the transcriptome in distal neurites. To investigate this, we micro-dissected soma and axons from the corpus callosum of P0 mouse brains and conducted RNA-seq on both WT and Nestin-Cre Mettl14 conditional knockout mice. We found that m6A modification positively promotes the neurite localization of a subset of mRNAs related to axonogenesis and growth cone regulation, which is essential for normal axonogenesis.

ORGANISM(S): Mus musculus

PROVIDER: GSE277230 | GEO | 2026/01/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

3' mRNA-seq of neurites and soma isolated from mESC-derived neurons

Project description:3' mRNA-seq was performed with QuantSeq 3' mRNA-Seq kit (Lexogen 015) according to the manufacturer’s recommendations. 3' mRNA-seq was done in biological triplicates (soma) or duplicates (neurites), using 260 ng of total RNA from neurites or soma of mESC-derived neurons per sample. Libraries were pooled and sequenced on Illumina NextSeq 500 system with a single-end 150-cycle run.

2019-01-12 | E-MTAB-7251 | biostudies-arrayexpress

3' mRNA-seq of neurites and soma isolated from mESC-derived neurons

Project description:3' mRNA-seq of neurites and soma isolated from mESC-derived neurons

| PRJEB28860 | ENA

hnRNPR interactome in axons and soma of murine motoneurons

Project description:We determined the protein interaction partners of hnRNP R in murine motoneuron axons and soma by immunoprecipitation-MS. The neurons were grown in microchambers and axons and soma could be harvested individually. We performed an IP with an antibody against the murine hnRNP R.

2024-08-22 | PXD043851 | Pride

RNA-seq in neurites and soma of primary hippocampal neurons isolated from P0 or P1 mice

Project description:RNA-seq in neurites and soma of primary hippocampal neurons isolated from P0 or P1 mice

| PRJNA834991 | ENA

Neuronal zipcode identification protocol (N-zip) in primary cortical neurons

Project description:For identification of transcripts enriched in neurites of primary cortical neurons, the cells were plated on a microporous membrane for isolation of neurites and soma. Extracted RNA was used for preparation of mRNA-seq, total RNA-seq or smRNA-seq libraries and Illumina sequencing. For neuronal zipcode identification protocol (N-zip) in mouse cortical neurons, we combined a massively parallel reporter assay with neurites/soma separation. Neurons, grown on a microporous membrane, were infected with a library of around 5000 oligos tiled across 3'UTRs of selected neurite-enriched transcripts, cloned downstream of GFP coding sequence. RNA was extracted from soma and neurites and reverse transcribed into cDNA. Amplicon libraries of 3'UTR reporters were prepared and subjected to Illumina sequencing. In the second round of N-zip, a library containing selected reporters from the first N-zip and their mutagenized versions (around 6000 oligos) were used. In the following rounds, N-zip was combined with knockdown of selected genes.

2022-12-02 | E-MTAB-10902 | biostudies-arrayexpress

Profiling of soma and neurite transcriptomes

Project description:We report mRNA profiles of subcellularly localized transcriptomes (soma and neurite) of two mouse cell lines, N2A and CAD, as well as primary cortical neurons from E18.5 mice. We also performed this fractionation and sequencing after RNAi knockdown (cell lines) or in knockout mice (primary cortical neurons) of the RNA-binding proteins muscleblind 1 and 2 (Mbnl1 and Mbnl2). Fractionate neurons using porous transwell membranes. Isolate poly-A RNA.

2016-02-19 | E-GEOD-67828 | biostudies-arrayexpress

RNA-seq of neuronal compartments with perturbed mRNA degradation machinery

Project description:We have perturbed mRNA degradation machinery and investigated the change in subcellular localization of mRNA in Ascl1-induced neurons (iNeurons). Mutant iNeuron line harbouring a ponasteroneA-inducible heterozygous dominant-negative Caf1 (dnCaf1) was generated, separated into neuronal compartments (soma and neurites) and sequenced in parallel with compartments from the Wild Type iNeurons (WT). Differential localization of mRNA between soma and neurites in the WT was then compared to the localization in dnCaf1 in order to identify the changes in localization.

2023-12-31 | E-MTAB-9398 | biostudies-arrayexpress

SlamSeq of subcellular compartments in mouse primary cortical neurons

Project description:We performed SlamSeq (thiol(SH)-linked alkylation for metabolic sequencing) to estimate mRNA half-lives in subcellular compartments (neurites, soma-cytoplasm and nucleus) of primary cortical neurons.

2023-07-12 | E-MTAB-12888 | biostudies-arrayexpress

Expression data from Soma dissected from single-cell mouse Hippocampal neurons

Project description:To assess neuronal expression divergence between mice and rats, we used the Affymetrix array platform to assay the transcriptomes of micro-dissected individual soma and pool of dendrites of hippocampal neurons in dispersed primary cell cultures from rat and mouse. Using microdissected soma and dendrites from primary cultures of hippocampal neurons of two mouse strains (C57BL/6 and Balb/c) and one rat strain (Sprague-Dawley), we investigate via microarrays, subcellular localization of mRNAs in neurons

2014-10-14 | E-GEOD-61084 | biostudies-arrayexpress

Expression data from Soma dissected from single-cell rat Hippocampal neurons

2014-10-14 | E-GEOD-61085 | biostudies-arrayexpress