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Medicago Mting1 Mting2 double knockout mutants are extremely dwarfed and never flower implicating essential MtING functions in growth and flowering

ABSTRACT: The regulation of flowering time to synchronise with external environmental signals is critical to plant reproductive success and agricultural productivity. Previously, we used CRISPR-Cas9 gene editing to show the INHIBITOR OF GROWTH 2 gene (MtING2) promotes flowering and growth in the model temperate legume Medicago truncatula, especially under inductive vernalized long days. A second ING gene, MtING1, did not appear to regulate flowering. To further dissect the genetic function of the two ING genes in flowering and growth, we cross-pollinated selected Mting1 and Mting2 single mutants to create two different double mutants; Mting1-7 Mting2-2 double knockout mutant and Mting1-1 Mting2-11 double PHD finger mutant. The growth and flowering of these mutants was assessed in vernalized long-day conditions. We also used fluorescence confocal microscopy and in vitro protein biophysical analysis to investigate the subcellular localization and oligomerization of the proteins. Finally we carried out gene expression analysis by both RNA-seq and RT-qPCR to determine how the two genes affect transcript accumulation to influence flowering.

ORGANISM(S): Medicago truncatula

PROVIDER: GSE277907 | GEO | 2025/04/09

REPOSITORIES: GEO

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Medicago Mting1 Mting2 double knockout mutants are extremely dwarfed and never flower implicating essential MtING functions in growth and flowering

Project description:Medicago Mting1 Mting2 double knockout mutants are extremely dwarfed and never flower implicating essential MtING functions in growth and flowering

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