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Single cell mRNA sequencing of Peyer's patch LysoDCs and cDC2s and blood CD45+CD115hi cells

ABSTRACT: We investigated the genetic relatedness between CD115hi blood cell subsets, LysoDCs and Peyer's patch cDC2s, a close but ontogenically unrelated phagocyte subset. We isolated Ly6ChiCD43-, Ly6CloCD43hi and Ly6C-CD43-CD11b+SIRPa+ cells from CD115hi blood cells of 9-day clodronate-treated mice, when the latter were in higher number than non-treated mice. In parallel, we sorted cDC2s and the four differentiation states of LysoDCs from PPs of non-treated mice. scRNAseq analysis of these populations allowed to identify the blood precursors of LysoDCs.

ORGANISM(S): Mus musculus

PROVIDER: GSE278468 | GEO | 2026/01/31

REPOSITORIES: GEO

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Single cell mRNA sequencing of Peyer's patch LysoDCs and cDC2s and blood CD45+CD115hi cells

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