Transcriptomics

Dataset Information

24

Long non-coding RNAs detected in Plasmodium falciparum malaria using high-resolution DNA tiling microarray technology.


ABSTRACT: Survey of transcriptional activity across 22.6% of the P. falciparum strain 3D7 genome. 872 protein coding genes and 60 putative P. falciparum lncRNAs identified under developmental regulation during the parasite's pathogenic human blood stage. Overall design: Total RNA was isolated from synchronous in vitro parasite cultures at 18, 24, 30, and 36 hours post invasion (hpi) of red blood cells. 1 μg of total RNA was then subjected to poly-A selective amplification using Message Amp II (Ambion), substituting biased dNTP/NTP mixes (2A/T/U:1C/G). The resulting aRNA was labeled with Superscript II reverse transcriptase (Invitrogen), using random hexamers, and either Cy3- or Cy5-dUTPs (Amersham).

INSTRUMENT(S): Rinn Sabeti P. falciparum Tiling 385k v1.0

ORGANISM(S): Plasmodium falciparum 3D7  

SUBMITTER: Kate Mariel Broadbent  

PROVIDER: GSE27937 | GEO | 2011-03-16

SECONDARY ACCESSION(S): PRJNA137865

REPOSITORIES: GEO

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