Project description:Expression profiling of cultured HL-1 cardiomyocytes subjected to hypoxia for 8 hours. Three samples from each condition were analyzed
Project description:Analysis of heart of Hypoxia-inducible 2 alpha-deficient mice, specific in cardiomyocytes (Hif2aloxP/loxP Myosin Cre+) subjected to myocardial ishcemia reperfusion. Hif2a belongs to the family of transcription factors, that regulate a transcriptional response to tissue hypoxia.
Project description:In this study, we performed temporal profiling of transcriptome and chromatin accessibility in HL-1 cells for understanding the molecular mechanisms underlying cardiac responses to hypoxia. We collected HL-1 cells under four conditions (4 h and 8 h of hypoxia exposure, 24 h reoxygenation and the normal condition), applied RNA-seq and ATAC-seq to them and performed pairwise comparison of gene expression and open chromatin status on a genome-wide scale.
Project description:RNA sequencing and subsequent bioinformatics analyses were performed at early reoxygenation stages in HL-1 cardiomyocytes treated or not with BRL37344 Methods: HL-1 cardiomyocytes were subjected to Hypoxia/Reoxygenation (6h/1h) with/without a M-NM-23AR agonist (BRL37344 5M-BM-5mol/L). mRNA profiles were generated by deep sequencing, in triplicate, using Illumina GAIIx.The sequence reads that passed quality filters were quantified using BWA aligned reads using RSEM. qRTM-bM-^@M-^SPCR validation was performed using SYBR Green assay. Results: After 6h of hypoxia followed by 1h reoxygenation, 866 genes were differentially expressed upon M-NM-23AR stimulation by BRL37344. Among these, 177 were at least 2-fold up or downregulated. Conclusions: Our results show that Hsp70 plays a key role in the cardioprotection afforded by M-NM-23AR agonism in cardiomyocytes during the early window of H/R. mRNA profiles from cardiomyocyte subjected to H/R (6h/1h) with/without a M-NM-23AR agonist were generated by deep sequencing, in triplicate, using Illumina GAIIx.
Project description:RNA sequencing and subsequent bioinformatics analyses were performed at early reoxygenation stages in HL-1 cardiomyocytes treated or not with BRL37344 Methods: HL-1 cardiomyocytes were subjected to Hypoxia/Reoxygenation (6h/1h) with/without a β3AR agonist (BRL37344 5µmol/L). mRNA profiles were generated by deep sequencing, in triplicate, using Illumina GAIIx.The sequence reads that passed quality filters were quantified using BWA aligned reads using RSEM. qRT–PCR validation was performed using SYBR Green assay. Results: After 6h of hypoxia followed by 1h reoxygenation, 866 genes were differentially expressed upon β3AR stimulation by BRL37344. Among these, 177 were at least 2-fold up or downregulated. Conclusions: Our results show that Hsp70 plays a key role in the cardioprotection afforded by β3AR agonism in cardiomyocytes during the early window of H/R.
Project description:Data from iPSC-derived cardiomyocytes from three different individuals treated with either 0.5 uM Doxorubicin or vehicle for 24 hours. The treatment was replicated three times for one of the individuals. Protein was extracted from the ten samples and subjected to NanoLC MS/MS Analysis with data-independent acquisition.
Project description:Postnatal day 4 neonatal rat cardiomyocytes transduced with LacZ or flag-tagged, activated YAP1 (S127A) expressing adenovirus After transduction, cells were cultured in serum free media and collected 48 hours later.
Project description:We hypothesize that cultured macrophages directly exposed intermittent hypoxia will have a greater change in expression in genes related to inflammatory response than macrophages exposed to sustained hypoxia. We will evaluate gene expression using microarray analysis of RNA collected from RAW 264.7 macrophages cultured under the following environmental conditions: 1) 4 hours of intermittent hypoxia (2 minute cycles: 90 seconds at 40 Torr and 30 seconds at 8 Torr), 2) 4 hours of sustained hypoxia (8 Torr), and 3 ) standard tissue culture conditions (141 Torr; reference).
Project description:We hypothesize that the culture media collected from macrophages exposed to intermittent hypoxia will induce a greater pro-inflammatory gene profile in naïve cultured macrophages than will culture media collected from macrophages exposed to sustained hypoxia. We will evaluate gene expression using microarray analysis of RNA collected from RAW 264.7 macrophages cultured for 24 hours in DMEM media obtained from 1) cells cultured with intermittent hypoxia (2 minute cycles: 90 seconds at 40 Torr and 30 seconds at 8 Torr), 2) media exposed to intermittent hypoxia, 3) cells cultured with sustained hypoxia (8 Torr), 4) media exposed to sustained hypoxia and 4) standard tissue culture conditions (fresh DMEM media; reference).
Project description:Wild-type, NOTCH1-knockdown, and TP53-knockdown MDA-MB-231 cells were cultured under normoxia, hypoxia, oscillatory hypoxia, and lactate treatment, and subjected to RNA sequencing to assess transcriptional changes.