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Uncovering changes in 3D-chromatin structure and dynamic gene expression during spermatogenesis [RNA-seq]

ABSTRACT: We conducted a high-throughput analysis of the chromatin structural organisation and gene expression by using porcine spermatogonia (SPG), spermatocytes (SPY) and round spermatids (RS). We found that during spermatogenesis, SPY showed a weaker pattern of chromosomal interactions, attenuated compartmentalisation and a reduction in the number of TADs, which was restored during the subsequent period of round spermatids. This suggests a reprogramming of chromatin higher-order structures during porcine spermatogonia differentiation. Our results reveal that chromatin structure changes during porcine spermatogenesis, along with changes in gene expression.

ORGANISM(S): Sus scrofa

PROVIDER: GSE280547 | GEO | 2025/10/23

REPOSITORIES: GEO

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Uncovering changes in 3D-chromatin structure and dynamic gene expression during spermatogenesis [Hi-C]

Project description:We conducted a high-throughput analysis of the chromatin structural organisation and gene expression by using porcine spermatogonia (SPG), spermatocytes (SPY) and round spermatids (RS). We found that during spermatogenesis, SPY showed a weaker pattern of chromosomal interactions, attenuated compartmentalisation and a reduction in the number of TADs, which was restored during the subsequent period of round spermatids. This suggests a reprogramming of chromatin higher-order structures during porcine spermatogonia differentiation. Our results reveal that chromatin structure changes during porcine spermatogenesis, along with changes in gene expression.

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