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RNF26 promote the proliferation and metastasis of colorectal cancer cells via Wnt pathway

ABSTRACT: Colorectal cancer (CRC) is the third most common malignant tumor in the world. Identification of key genes for prediction and treatment of disease course and prognosis is urgent to reduce morbidity and mortality of CRC patients. Although dysregulated Ring finger protein 26 (RNF26) expression has been detected in several tumors, the potential regulatory effect of RNF26 on CRC remains uncertain. Here we evaluated the expression of CRC using multiple online platforms, and demonstrated that RNF26 upregulation was consistently observed in CRC relative to normal tissues and correlated with unfavorable prognosis. Then, we provide direct evidence that RNF26 acts as a driver of CRC progression by showing that constructed overexpression cell lines. We analyzed the impact of altering RNF26 level on CRC cell viability and metastasis phenotypes in HCT116 and HCT15 modified cell lines. The results showed that RNF26 overexpression promote cell proliferation, migration, and invasion in vitro, and multiple organ distant metastasis in vivo. Moreover, Wnt signaling pathway was common enriched in these modified cell lines. Therefore, we assumed that RNF26 significantly increased CRC proliferation and metastasis through the RNF26/Wnt axis. These data strongly suggest that RNF26 overexpression may be an effective biomarker to monitor and a prospective therapeutic target to treat CRC.

ORGANISM(S): Homo sapiens

PROVIDER: GSE280645 | GEO | 2025/04/30

REPOSITORIES: GEO

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Project description:Background Colorectal cancer (CRC), a malignancy with high incidence and mortality rates, presents particularly severe prognoses at the stages of invasion and metastasis despite significant advancements in diagnostic and therapeutic technologies. The tumour microenvironment, especially the extracellular matrix (ECM) stiffness, has garnered considerable attention for its impact on CRC progression and metastasis. However, the precise relationship and molecular mechanisms between ECM stiffness and CRC prognosis remain unclear. Methods This study included 107 CRC patients. Tumor stiffness was assessed using magnetic resonance elastography (MRE), and collagen content was analysed with Masson staining. CRC cell lines SW480 and HCT116 were cultured on matrices of varying stiffness, followed by transcriptome sequencing to identify stiffness-related differential genes and investigate their functions and signalling pathways. Additionally, an HSF4 knockout CRC cell model was constructed to evaluate the effects of HSF4 on cell proliferation, migration, and invasion through both in vitro and in vivo experiments, exploring its role in tumour growth and metastasis. Results The study found that CRC tumour stiffness was significantly higher than normal tissue and positively correlated with collagen content and TNM staging. Transcriptome analysis revealed that high-stiffness matrices significantly regulated cell functions and signalling pathways. High expression of HSF4 was strongly associated with tumour stiffness and poor prognosis. HSF4 expression increased with higher TNM stages in CRC tissues, and its knockout significantly inhibited cell proliferation, migration, and invasion, particularly on high-stiffness matrices. In vivo experiments further confirmed that HSF4 promoted tumour growth and metastasis, independent of collagen protein increase. Conclusion This study reveals a close relationship between tumour stiffness and CRC prognosis, identifying HSF4 as a critical player in CRC progression. HSF4 promotes tumour proliferation and metastasis by regulating EMT-related signalling pathways, with its high expression closely linked to tumour stiffness. Although LOXL1 increased collagen content and a-SMA expression, HSF4's role in promoting tumour growth and metastasis was independent of collagen protein. HSF4 holds potential as a target for CRC prognosis evaluation and treatment. Future research should further explore the regulatory mechanisms of HSF4 and its potential for clinical application.

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RNF26 promote the proliferation and metastasis of colorectal cancer cells via Wnt pathway

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